School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China.
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China.
Int J Biol Macromol. 2018 Oct 1;117:42-50. doi: 10.1016/j.ijbiomac.2018.05.145. Epub 2018 May 23.
Insect pheromone biosynthesis activating neuropeptide (PBAN) controls the synthesis and actuating of sex pheromones of female adult. In the current examination, the full-length cDNA encoding the PBAN receptor was cloned from the pheromone gland (PG) of Antheraea pernyi (AntpePBANR). The AntpePBANR displayed the characteristic seven transmembrane areas of the G protein-coupled receptor (GPCR) and was closely related to the PBANR from Bombyx mori and Manduca sexta in the phylogenetic tree. The AntpePBANR expressed in mammalian cell lines were enacted by AntpePBAN in a concentration-dependent manner. AntpePBANR activation resulted in the calcium mobilization but did not activate the cAMP elevation pathway. Cells expressing AntpePBANR were profoundly responsive to Antpe-γ-SGNP (suboesophageal ganglion neuropeptides) and Antpe-DH (diapause hormone), different individuals from FXPRLamide (X = T, S or V) family in A. pernyi. Deletion of residues in the C-terminal hexapeptide (FSPRLamide) proved that P, R and L played the key parts in initiating the AntpePBANR, the amination to the last C terminal residues which can also likewise impact the activation of AntpePBAN receptor altogether. The mRNA of the AntpePBANR gene demonstrated the most noteworthy transcript levels in pheromone gland followed by fat body.
昆虫信息素生物合成激活神经肽（PBAN）控制雌性成虫性信息素的合成和作用。在当前的研究中，从柞蚕（AntpePBANR）的香腺中克隆出编码 PBAN 受体的全长 cDNA。AntpePBANR 显示出 G 蛋白偶联受体（GPCR）的特征七跨膜区，并且在系统发育树中与来自家蚕和烟草天蛾的 PBANR 密切相关。在哺乳动物细胞系中表达的 AntpePBANR 以浓度依赖的方式被 AntpePBAN 激活。AntpePBANR 的激活导致钙动员，但不会激活 cAMP 升高途径。表达 AntpePBANR 的细胞对 Antpe-γ-SGNP（食管下神经节神经肽）和 Antpe-DH（滞育激素）反应强烈，Antpe-DH 与 A. pernyi 中的 FXPRLamide（X= T、S 或 V）家族的不同个体。C 末端六肽（FSPRLamide）残基的缺失证明了 P、R 和 L 在启动 AntpePBANR 中起着关键作用，最后 C 末端残基的氨化也可以共同影响 AntpePBAN 受体的激活。AntpePBANR 基因的 mRNA 在香腺中表现出最高的转录水平，其次是脂肪体。
