Yadollahpour Ali, Rezaee Zohre, Bayati Vahid, Tahmasebi Birgani Mohammad Javad, Negad Dehbashi Fereshteh
Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Email:
Asian Pac J Cancer Prev. 2018 May 26;19(5):1259-1262. doi: 10.22034/APJCP.2018.19.5.1259.
Background: The efficiency of radiotherapy for tumors can be enhanced with different radiosensitizers. Previous studies have shown that electroporation (EP) can sensitize some cancer cell lines to ionizing radiation (IR). HT-29 is a radiation resistant colorectal cancer cell line, representative of a cancer type which is the second cause of cancer mortalities in developed countries. The present study aimed to evaluate radiosensitizing effects of EP on HT-29 cells in vitro exposed to 6 MV X-ray photon beams. Methods: HT-29 cells were exposed to a 6 MV X-ray photon beam as the control or to a combination of electroporation and irradiation. The response of cells was evaluated by colony formation assay and survival curves. Results: The survival fraction of the HT-29 cells was significantly decreased by electroporation prior to radiotherapy. A single electric pulse increased colorectal HT-29 cancer cell sensitivity to megavoltage radiation by a factor of 1.36. Conclusion: Our findings showed that EP before radiotherapy can significantly enhance tumor cell sensitivity. This combined treatment modality should be assessed for its applicability in clinic settings for employment against radioresistant cancers. However, to facilitate achieving this goal, many different tumors with a broad range of radiosensitivities should be evaluated.
使用不同的放射增敏剂可提高肿瘤放疗的效率。先前的研究表明，电穿孔（EP）可使某些癌细胞系对电离辐射（IR）敏感。HT-29是一种抗辐射的结肠癌细胞系，代表了在发达国家中癌症死亡率位居第二的癌症类型。本研究旨在评估电穿孔对体外暴露于6 MV X射线光子束的HT-29细胞的放射增敏作用。方法：将HT-29细胞作为对照暴露于6 MV X射线光子束，或暴露于电穿孔与照射的联合处理。通过集落形成试验和存活曲线评估细胞的反应。结果：放疗前进行电穿孔显著降低了HT-29细胞的存活分数。单个电脉冲使结肠HT-29癌细胞对兆伏级辐射的敏感性提高了1.36倍。结论：我们的研究结果表明，放疗前进行电穿孔可显著提高肿瘤细胞的敏感性。应评估这种联合治疗方式在临床环境中对抗辐射癌症的适用性。然而，为便于实现这一目标，应评估许多不同放射敏感性的肿瘤。
