Ito-Ishida Aya, Yamalanchili Hari Krishna, Shao Yingyao, Baker Steven A, Heckman Laura D, Lavery Laura A, Kim Ji-Yoen, Lombardi Laura M, Sun Yaling, Liu Zhandong, Zoghbi Huda Y
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX, USA.
Nat Neurosci. 2018 Jun;21(6):794-798. doi: 10.1038/s41593-018-0155-8. Epub 2018 May 25.
Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.
先前的研究表明，MeCP2 与连接组蛋白 H1 相互竞争，但这一假设从未在体内得到验证。在此，我们在小鼠前脑兴奋性神经元中对 Flag 标签标记的 H1.0 进行了染色质免疫沉淀测序（ChIP-seq）。出乎意料的是，Flag-H1.0 和 MeCP2 占据相似的基因组区域，且在 MeCP2 缺失时 Flag-H1.0 的结合并未改变。此外，H1.0 的轻度过表达并未改变 MeCP2 的结合，这表明 MeCP2 和 H1.0 的功能结合在很大程度上是独立的。
