Laboratory for Bone Metabolism, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China; Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
Bioorg Chem. 2018 Sep;79:334-340. doi: 10.1016/j.bioorg.2018.04.018. Epub 2018 May 3.
Three cationic lipids derived from [12]aneN modified with naphthalimide (1a), oleic acid (1b) and octadecylamine (1c) were designed and synthesized. In vitro transfection showed that all these liposomes can deliver plasmid DNA into the tested cell lines. Among these liposomes, 1a gave the best transfection efficiency (TE) in A549 cells, which was higher than that of lipofectamine 2000. More importantly, the TE of 1a was dramatically increased in the presence of 10% serum. These results suggested that 1a might be a promising non-viral gene vector, and also give further insight for developing novel high performance gene delivery agents.
设计并合成了三种阳离子脂质体，它们是由[12]烷胺（1a）、油酸（1b）和十八胺（1c）修饰的萘酰亚胺衍生而来。体外转染表明，所有这些脂质体都可以将质粒 DNA 递送到被测试的细胞系中。在这些脂质体中，1a 在 A549 细胞中表现出最好的转染效率（TE），高于脂质体 2000。更重要的是，在 10%血清存在的情况下，1a 的 TE 显著增加。这些结果表明，1a 可能是一种很有前途的非病毒基因载体，并为开发新型高性能基因传递剂提供了进一步的思路。
