Peoples' Friendship University of Russia,Mikluho-maklaya st.6, Moscow, 117198, Russia.
A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russia; Peoples' Friendship University of Russia,Mikluho-maklaya st.6, Moscow, 117198, Russia.
J Chromatogr A. 2018 Jul 27;1560:97-103. doi: 10.1016/j.chroma.2018.05.025. Epub 2018 May 23.
A simple and convenient on-spot derivatization has been suggested for the modification of hydroxyl-containing compounds for their analysis by thin layer chromatography/matrix-assisted laser desorption ionization mass spectrometry (TLC/MALDI). The proposed approach was based on post-chromatographic acylation of separated analytes by 3-bromopropionyl chloride with simultaneous quaternization of pyridine. In contrast to the initial alcohols not ionizable in TLC/MALDI conditions, the derivatives, containing permanent positive charge, revealintense peaks of their cationic moieties in MALDI mass spectra recorded directly from TLC plates. The method was tested on a series of mammalian and plant sterols, phenols and terpene alcohols.
一种简单便捷的现场衍生化方法被提出，用于对含羟基化合物进行修饰，以便通过薄层色谱/基质辅助激光解吸电离质谱法（TLC/MALDI）进行分析。所提出的方法基于 3-溴丙酰氯对分离的分析物进行的色谱后酰化，同时用吡啶季铵化。与初始醇类在 TLC/MALDI 条件下不可电离的情况相比，含有永久正电荷的衍生物在直接从 TLC 板记录的 MALDI 质谱中显示出其阳离子部分的强烈峰。该方法在一系列哺乳动物和植物甾醇、酚类和萜烯醇上进行了测试。
