Esparza Cesar, Borisov R S, Varlamov A V, Zaikin V G
People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russia.
People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russia; A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russia.
J Chromatogr A. 2016 Oct 28;1470:118-122. doi: 10.1016/j.chroma.2016.09.075. Epub 2016 Oct 4.
New composite matrices have been suggested for the analysis of mixtures of different synthetic organic compounds (N-containing heterocycles and erectile dysfunction drugs) by thin layer chromatography/matrix-assisted laser desorption ionization time-of-flight mass spectrometry (TLC/MALDI-TOF). Different mixtures of classical MALDI matrices and graphite particles dispersed in glycerol were used for the registration of MALDI mass spectra directly from TLC plates after analytes separation. In most of cases, the mass spectra possessed [M+H] ions; however, for some analytes only [M+Na] and [M+K] ions were observed. These ions have been used to generate visualized TLC chromatograms. The described approach increases the desorption/ionization efficiencies of analytes separated by TLC, prevent spot blurring, simplifies and decrease time for sample preparation.
已提出新型复合基质，用于通过薄层色谱/基质辅助激光解吸电离飞行时间质谱法（TLC/MALDI-TOF）分析不同合成有机化合物（含氮杂环和勃起功能障碍药物）的混合物。在分析物分离后，使用分散在甘油中的经典MALDI基质和石墨颗粒的不同混合物直接从TLC板上记录MALDI质谱。在大多数情况下，质谱具有[M+H]离子；然而，对于一些分析物，仅观察到[M+Na]和[M+K]离子。这些离子已用于生成可视化的TLC色谱图。所描述的方法提高了通过TLC分离的分析物的解吸/电离效率，防止斑点模糊，简化并减少了样品制备时间。
