Metabolomics and Mass Spectrometry Research Group, Amazonas State University - UEA, 690065-130 Manaus, AM, Brazil; Department of Chemistry, Federal University of Amazonas - UFAM, 69080-900 Manaus, AM, Brazil.
Department of Chemistry, Federal University of Amazonas - UFAM, 69080-900 Manaus, AM, Brazil.
Food Res Int. 2018 Jul;109:112-119. doi: 10.1016/j.foodres.2018.04.019. Epub 2018 Apr 14.
Remela de cachorro (Clavija lancifolia Desf.) is an Amazonian native fruit consumed specially in the Purus microregion. Because of its rarity, restricted consumption, and the lack of knowledge about its chemical composition, remela de cachorro fruit was studied in relation to its phenolic and aroma constitution. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), 11 compounds (flavonoids and its glucosides along with organic acids) were tentatively identified by fragmentation patterns. A previously validated method was applied to quantify common antioxidant compounds in the raw pulps, for which kaempferol was the main compound. Gas chromatography mass spectrometry (GC-MS) with headspace solid-phase microextraction (HS-SPME) was employed to assess the aroma composition of remela de cachorro fruit. A total of 27 volatile organic compounds (VOCs) were identified for this fruit, for which benzaldehyde and linalool were the main VOCs. Furthermore, biological activities, such as antioxidant capacity (ABTS, DPPH, and ORAC methods), cytotoxicity, and α-glucosidase and lipase inhibitions of the hydroalcoholic extract of remela de cachorro fruit were evaluated. In vitro biological assays revealed the potential of this fruit as a bioactive food that should be further studied and explored in Amazonian products.
狗果（Clavija lancifolia Desf.）是一种亚马逊原生水果，特别是在普鲁斯微地区被消费。由于其稀有性、限制消费以及对其化学成分缺乏了解，因此对狗果的果实进行了研究，涉及到其酚类和香气成分。使用液相色谱串联质谱（LC-MS/MS），通过碎片模式初步鉴定了 11 种化合物（类黄酮及其糖苷以及有机酸）。应用先前验证的方法来定量原始果肉中的常见抗氧化化合物，其中山柰酚是主要化合物。采用顶空固相微萃取（HS-SPME）与气相色谱-质谱联用（GC-MS）评估狗果的香气成分。确定了这种水果共 27 种挥发性有机化合物（VOCs），其中苯甲醛和芳樟醇是主要的 VOCs。此外，还评估了狗果水醇提取物的抗氧化能力（ABTS、DPPH 和 ORAC 方法）、细胞毒性以及α-葡萄糖苷酶和脂肪酶抑制活性。体外生物测定表明，这种水果具有作为生物活性食品的潜力，应在亚马逊地区的产品中进一步研究和探索。
