Rogers B J
Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Reprod Toxicol. 1988;2(3-4):233-40. doi: 10.1016/0890-6238(88)90029-9.
The sperm penetration assay (SPA), which involves the use of zona-free hamster eggs to assess fertilizing potential of human spermatozoa, has the capability of evaluating the functional capacity of sperm. If men are exposed to a toxic insult that could result in impaired fertility, this has been monitored historically by determining the routine semen parameters of concentration, motility, and morphology. The lack of availability of the SPA for evaluating toxic exposure in field studies has hampered its utilization. The purpose of this article is to illustrate the use of the SPA in a specific field study and to describe two recent innovations in the SPA that make its use more accessible for field studies. These two innovations are shipping semen samples in TEST-yolk buffer by overnight express to a central laboratory for testing and using frozen hamster eggs in the test. The report is divided into the following three major sections: I. Kunia study; II. TEST-yolk buffer modifications of SPA; and III. Use of frozen eggs in SPA.
精子穿透试验（SPA），即使用去透明带仓鼠卵来评估人类精子的受精潜力，具有评估精子功能能力的作用。如果男性受到可能导致生育能力受损的毒性侵害，以往是通过测定精液常规参数（浓度、活力和形态）来监测的。在现场研究中缺乏用于评估毒性暴露的SPA，这阻碍了其应用。本文的目的是说明SPA在一项特定现场研究中的应用，并描述SPA最近的两项创新，这使得它在现场研究中更易于使用。这两项创新是通过隔夜快递将精液样本装在TEST-蛋黄缓冲液中运往中央实验室进行检测，以及在试验中使用冷冻仓鼠卵。报告分为以下三个主要部分：一、库尼亚研究；二、SPA的TEST-蛋黄缓冲液改良；三、SPA中冷冻卵的使用。
