Iguchi T, Irisawa S, Uchima F D, Takasugi N
Department of Biology, Yokohama City University, Japan.
Reprod Toxicol. 1988;2(2):127-34. doi: 10.1016/0890-6238(88)90008-1.
Male and female C57BL/Tw mice were given 5 daily subcutaneous injections of 100 micrograms tamoxifen (Tx), starting on the day of birth (Tx mice). In untreated fetal mice on day 18 of gestation, the greater part of the pubic and ischial bones were cartilaginous. At more than 30 days of age, however, untreated mice showed completely calcified pelvic bone, whereas in age-matched Tx mice the greater part of the junctional regions in the pelvis remained cartilaginous. Treatment with Tx starting within 5 days of age caused bladder hernia with or without cecum hernia. The pubic ligament in Tx mice at ages of 30-540 days was markedly expanded as compared with that in age-matched controls. The permanent chondrification in the pelvis was found in all mice given Tx starting within 10 days of age. By contrast, neonatal treatments of mice with other antiestrogens, clomiphene and nafoxidine (100 micrograms/day), induced neither permanent chondrification in the pelvis nor expansion of the pubic ligament nor hernia. These findings suggest that Tx has a specific effect on the symphysis pubis and some junctional regions of the developing pelvis in mice when given neonatally.
从出生日起，对雄性和雌性C57BL/Tw小鼠每日进行5次皮下注射，每次注射100微克他莫昔芬（Tx）（Tx小鼠）。在妊娠第18天未经处理的胎鼠中，耻骨和坐骨的大部分为软骨组织。然而，在30多天龄时，未经处理的小鼠骨盆骨完全钙化，而在年龄匹配的Tx小鼠中，骨盆连接区域的大部分仍为软骨组织。在出生后5天内开始用Tx治疗会导致膀胱疝，伴有或不伴有盲肠疝。与年龄匹配的对照组相比，30 - 540天龄的Tx小鼠耻骨韧带明显增宽。在出生后10天内开始给予Tx的所有小鼠中，均发现骨盆永久性软骨化。相比之下，用其他抗雌激素药物氯米芬和萘福昔定（100微克/天）对新生小鼠进行治疗，既未诱导骨盆永久性软骨化，也未导致耻骨韧带增宽或疝形成。这些发现表明，新生期给予Tx对小鼠耻骨联合和发育中骨盆的一些连接区域具有特定作用。
