Xue Y, Nie M, Wang O, Wang C Y, Han G Y, Shen Q, Deng H Y, Jiang Y, Li M, Xia W B, Xing X P, Xu L
Department of Endocrinology, Key Laboratory of Endocrinology, National Health and Family Planning Commission, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi. 2018 May 15;98(18):1408-1413. doi: 10.3760/cma.j.issn.0376-2491.2018.18.008.
To explore the association between α-actinin-3 (3) polymorphism and muscle strength in postmenopausal women. Five hundred and ninety-eight postmenopausal women with an average of (62.9±7.0) years old in Dongcheng District of Beijing were included. The 3 polymorphism including rs540874, rs618838 and rs2229456 were genotyped by Sequenom Mass Array to explore their associations with muscle strength. One hundred and sixty-three of them were trained with regular Tai chi movement while 271 were administered with elemental calcium 600 mg/d combined with Vitamin D 800 U/d or calcitriol 0.25 μg/d for 2 years. Association between changes of muscle strength and 3 polymorphism were analyzed. The rs540874 genotypes were found to be significantly associated with chair stand test[GG (9.02±3.85) s vs GA (9.27±4.14) s vs AA (9.68±5.00) s, =0.015]. Right grip strength in women with G allele were likely to be higher compared with A allele, but it was not statistically significant (=0.056). Multiple linear regression showed that the chair stand test of AA genotype was statistically longer than that of GG and GA genotype (β=2.639, 95% : 1.632-4.646, =0.010). The associations between rs618838, rs2229456 genotypes and muscle strength of both lower and upper limbs were not significant (all >0.05). In addition, muscle strength of lower limbs of patients with rs540874 genotyped with G allele, rs618838 genotyped with C allele and rs2229456 genotyped with A allele increased significantly after enhanced exercise and vitamin D supplementation (all <0.05). The rs540874 polymorphism of 3 gene was associated with the muscle function of lower limb in postmenopausal women. The improvement of muscle strength after intervention were possibly correlated with rs540874, rs618838 and rs2229456 polymorphisms.
探讨α-辅肌动蛋白-3（ACTN3）基因多态性与绝经后女性肌肉力量之间的关联。纳入了北京市东城区598名平均年龄为（62.9±7.0）岁的绝经后女性。采用Sequenom Mass Array技术对包括rs540874、rs618838和rs2229456在内的ACTN3基因多态性进行基因分型，以探讨它们与肌肉力量的关联。其中163人进行规律太极拳运动训练，271人每日服用600mg元素钙联合800U维生素D或0.25μg骨化三醇，持续2年。分析肌肉力量变化与ACTN3基因多态性之间的关联。发现rs540874基因型与椅子站立试验显著相关[GG型（9.02±3.85）秒 vs GA型（9.27±4.14）秒 vs AA型（9.68±5.00）秒，P = 0.015]。携带G等位基因的女性右手握力可能高于携带A等位基因的女性，但差异无统计学意义（P = 0.056）。多元线性回归显示，AA基因型的椅子站立试验时间在统计学上长于GG和GA基因型（β = 2.639，95%置信区间：1.632 - 4.646，P = 0.010）。rs618838、rs2229456基因型与上下肢肌肉力量之间的关联均无统计学意义（均P > 0.05）。此外，rs540874基因分型为G等位基因、rs618838基因分型为C等位基因以及rs2229456基因分型为A等位基因的患者，在进行强化运动和补充维生素D后下肢肌肉力量显著增加（均P < 0.05）。ACTN3基因的rs540874多态性与绝经后女性下肢肌肉功能相关。干预后肌肉力量的改善可能与rs540874、rs618838和rs2229456基因多态性有关。
