Itaka Toshio, Agemizu Kenichiro, Aruga Seiji, Machida Shuichi
1Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan; 2School of Physical Education, Tokai University, Kanagawa, Japan; and 3Sports Medical Science Research Institute, Tokai University, Kanagawa, Japan.
J Strength Cond Res. 2016 Jul;30(7):2043-8. doi: 10.1519/JSC.0000000000001300.
Itaka, T, Agemizu, K, Aruga, S, and Machida, S. G allele of the IGF2 ApaI polymorphism is associated with judo status. J Strength Cond Res 30(7): 2043-2048, 2016-Previous studies have reported that the insulin-like growth factor 2 (IGF2) ApaI polymorphism is associated with body mass index, fat mass, and grip strength. Competitive judo requires high levels of strength and power. The purpose of this study was to investigate the association between the IGF2 ApaI and ACTN3 R577X polymorphisms and judo status. The subjects were 156 male judo athletes from a top-level university in Japan. They were divided into 3 groups based on their competitive history: international-level athletes, national-level athletes, and others. Genomic DNA was extracted from the saliva of each athlete, and the maximal isometric strength of the trunk muscles and handgrip strength were measured. Genotyping by polymerase chain reaction-restriction fragment length polymorphism was used to detect IGF2 (rs680) and α-actinin-3 (ACTN3) (rs1815739) gene polymorphisms. The genotype frequencies of the 2 gene polymorphisms were compared among the 3 groups of judo athletes and controls. International-level judo athletes showed a higher frequency of the GG + GA genotype of the IGF2 gene than that of the national-level athletes and others. There was an inverse linear correlation between the frequency of the IGF2 AA genotype and level of judo performance (p = 0.041). Back muscle strength relative to height and weight was higher in subjects with the GG + GA genotype than in those with the AA genotype. Conversely, the ACTN3 R577X polymorphism was not associated with judo status. Additionally, no differences were found in back muscle or handgrip strength among the ACTN3 genotypes. In conclusion, the results indicate that the IGF2 gene polymorphism may be associated with judo status.
板田敏、上水口健、荒贺史、町田司。胰岛素样生长因子2（IGF2）ApaI基因多态性的G等位基因与柔道水平相关。《力量与体能研究杂志》30(7): 2043 - 2048, 2016年 - 以往研究报道，胰岛素样生长因子2（IGF2）ApaI基因多态性与体重指数、体脂量和握力相关。竞技柔道需要高水平的力量和爆发力。本研究的目的是调查IGF2 ApaI和α -辅肌动蛋白3（ACTN3）R577X基因多态性与柔道水平之间的关联。研究对象为来自日本一所顶尖大学的156名男性柔道运动员。根据他们的比赛经历将其分为3组：国际水平运动员、国家水平运动员和其他运动员。从每位运动员的唾液中提取基因组DNA，并测量躯干肌肉的最大等长力量和握力。采用聚合酶链反应 - 限制性片段长度多态性基因分型法检测IGF2（rs680）和α -辅肌动蛋白3（ACTN3）（rs1815739）基因多态性。比较3组柔道运动员和对照组中这2种基因多态性的基因型频率。国际水平的柔道运动员中IGF2基因的GG + GA基因型频率高于国家水平运动员和其他运动员。IGF2基因AA基因型频率与柔道表现水平呈负线性相关（p = 0.041）。GG + GA基因型受试者相对于身高和体重的背部肌肉力量高于AA基因型受试者。相反，ACTN3 R577X基因多态性与柔道水平无关。此外，ACTN3各基因型之间在背部肌肉力量或握力方面未发现差异。总之，结果表明IGF2基因多态性可能与柔道水平相关。
