鱼鳞胶原蛋白（）对幼仓鼠肾成纤维细胞-21成纤维细胞培养的活力测试。
Viability test of fish scale collagen () on baby hamster kidney fibroblasts-21 fibroblast cell culture.
作者信息
Prahasanti Chiquita, Wulandari Denny Tri, Ulfa Noer
机构信息
Department of Periodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
出版信息
Vet World. 2018 Apr;11(4):506-510. doi: 10.14202/vetworld.2018.506-510. Epub 2018 Apr 18.
AIM
This study aims to examine the toxicity of collagen extracted from gouramy fish scales () by evaluating its viability against baby hamster kidney fibroblasts-21.
MATERIALS AND METHODS
Collagen was extracted from gouramy fish scales () with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment.
RESULTS
Collagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of0.754 (p>00025).
CONCLUSION
Collagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.
目的
本研究旨在通过评估从丝足鲈鱼鳞中提取的胶原蛋白对幼仓鼠肾成纤维细胞-21的活力，来检测其毒性。
材料与方法
用6%的醋酸从丝足鲈鱼鳞中提取胶原蛋白。使用傅里叶变换红外光谱和冷冻干燥技术对其结果进行分析。然后用扫描电子显微镜分析其形态。之后，进行3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐试验，以比较经鱼鳞胶原蛋白处理和未经处理的细胞。
结果
从丝足鲈鱼鳞中提取的胶原蛋白对培养的成纤维细胞无统计学影响，双侧统计显著性值为0.754（p>0.0025）。
结论
从丝足鲈鱼鳞中提取的胶原蛋白对BHK21成纤维细胞具有高活力。
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