Ahn Yun Gyong, Jeon So Hyeon, Lim Hyung Bae, Choi Na Rae, Hwang Geum-Sook, Kim Yong Pyo, Lee Ji Yi
Western Seoul Center, Korea Basic Science Institute, Seoul 03759, Republic of Korea.
Air Quality Research Division, National Institute of Environmental Research, Incheon 22689, Republic of Korea.
J Anal Methods Chem. 2018 Apr 1;2018:8341630. doi: 10.1155/2018/8341630. eCollection 2018.
Advanced separation technology paired with mass spectrometry is an ideal method for the analysis of atmospheric samples having complex chemical compositions. Due to the huge variety of both natural and anthropogenic sources of organic compounds, simultaneous quantification and identification of organic compounds in aerosol samples represents a demanding analytical challenge. In this regard, comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS) has become an effective analytical method. However, verification and validation approaches to quantify these analytes have not been critically evaluated. We compared the performance of gas chromatography with quadrupole mass spectrometry (GC-qMS) and GC×GC-TOFMS for quantitative analysis of eighteen target polycyclic aromatic hydrocarbons (PAHs). The quantitative obtained results such as limits of detection (LODs), limits of quantification (LOQs), and recoveries of target PAHs were approximately equivalent based on both analytical methods. Furthermore, a larger number of analytes were consistently identified from the aerosol samples by GC×GC-TOFMS compared to GC-qMS. Our findings suggest that GC×GC-TOFMS would be widely applicable to the atmospheric and related sciences with simultaneous target and nontarget analysis in a single run.
先进的分离技术与质谱联用是分析化学成分复杂的大气样品的理想方法。由于有机化合物的天然来源和人为来源种类繁多，同时对气溶胶样品中的有机化合物进行定量和鉴定是一项具有挑战性的分析任务。在这方面，全二维气相色谱与飞行时间质谱联用（GC×GC-TOFMS）已成为一种有效的分析方法。然而，尚未对定量这些分析物的验证和确认方法进行严格评估。我们比较了气相色谱与四极杆质谱联用（GC-qMS）和GC×GC-TOFMS对18种目标多环芳烃（PAHs）进行定量分析的性能。基于这两种分析方法，目标PAHs的定量结果，如检测限（LOD）、定量限（LOQ）和回收率大致相当。此外，与GC-qMS相比，GC×GC-TOFMS从气溶胶样品中一致鉴定出的分析物数量更多。我们的研究结果表明，GC×GC-TOFMS将广泛应用于大气及相关科学领域，能够在一次运行中同时进行目标分析和非目标分析。
