Institute of Chemistry, Universidade Estadual de Campinas, 270 Monteiro Lobato, Campinas, SP, 13083-970, Brazil.
National Institute of Science and Technology in Bioanalytics (INCTBio), Campinas, SP, 13083-970, Brazil.
Anal Bioanal Chem. 2018 Jul;410(19):4749-4762. doi: 10.1007/s00216-018-1134-3. Epub 2018 May 28.
In this study, a series of polymeric ionic liquid (PIL) sorbent coatings is evaluated for the extraction of polar volatile organic compounds (VOCs) from Brazilian wines using headspace solid-phase microextraction (HS-SPME), including samples from 'Isabella' and 'BRS Magna' cultivars-the latter was recently introduced by the Brazilian Agricultural Research Corporation - National Grape & Wine Research Center. The structurally tuned SPME coatings were compared to the commercial SPME phases, namely poly(acrylate) (PA) and divinylbenzene/carboxen/poly(dimethylsiloxane) (DVB/CAR/PDMS). The separation, detection and identification of the aroma profiles were obtained using comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). The best performing PIL-based SPME fiber, namely 1-hexadecyl-3-vinylimidazolium bis[(trifluoromethyl)sulfonyl]imide with 1,12-di(3-vinylimidazolium)dodecane dibis[(trifluoromethyl)sulfonyl]imide incorporated cross-linker supported on an elastic nitinol wire, exhibited superior performance to DVB/CAR/PDMS regarding the average number of extracted peaks and extracted more polar analytes providing additional insight into the aroma profile of 'BRS Magna' wines. Four batches of wine were evaluated, namely 'Isabella' and 'BRS Magna' vintages 2015 and 2016, using highly selective PIL-based SPME coatings and enabled the detection of 350+ peaks. Furthermore, this is the first report evaluating the aroma of 'BRS Magna' wines. A hybrid approach that combined pixel-based Fisher ratio and peak table-based data comparison was used for data handling. This proof-of-concept experiment provided reliable and statistically valid distinction of wines that may guide regulation agencies to create high sample throughput protocols to screen wines exported by Brazilian vintners. Graphical abstract Highly selective extraction of wine aroma using polymeric ionic liquid.
在这项研究中，评估了一系列聚合离子液体（PIL）吸附剂涂层，用于使用顶空固相微萃取（HS-SPME）从巴西葡萄酒中提取极性挥发性有机化合物（VOCs），包括'伊莎贝拉'和'BRSMagna'品种的样品 - 后者是由巴西农业研究公司 - 国家葡萄与葡萄酒研究中心最近推出的。结构调谐的 SPME 涂层与商业 SPME 相，即聚（丙烯酸酯）（PA）和二乙烯基苯/羧基/聚（二甲基硅氧烷）（DVB/CAR/PDMS）进行了比较。使用全二维气相色谱质谱联用（GC×GC-MS）获得香气轮廓的分离、检测和鉴定。表现最好的 PIL 基 SPME 纤维，即 1-十六烷基-3-乙烯基咪唑双（三氟甲基磺酰基）亚胺与 1,12-双（3-乙烯基咪唑）十二烷二双（三氟甲基磺酰基）亚胺交联剂结合在弹性镍钛合金丝上，在提取峰的平均数量和提取更多极性分析物方面表现优于 DVB/CAR/PDMS，为'BRS Magna'葡萄酒的香气特征提供了更多的见解。使用高度选择性的 PIL 基 SPME 涂层评估了四个批次的葡萄酒，即 2015 年和 2016 年的'伊莎贝拉'和'BRS Magna'年份，共检测到 350 多个峰。此外，这是首次评估'BRS Magna'葡萄酒香气的报告。结合基于像素的 Fisher 比和基于峰表的数据比较的混合方法用于数据处理。该概念验证实验提供了可靠且具有统计学意义的葡萄酒区分，可以指导监管机构创建高通量协议来筛选巴西酿酒商出口的葡萄酒。
