[Experimental study of urinary center change in pons after conus medullaris injury in rats].
作者信息
Yu Ronghua, Hou Chunlin, Lin Haodong, Zhao Jianguo, Zong Haiyang, Lin Yaofa
机构信息
Second Military Medical University, Shanghai, 200433, P.R.China;Department of Orthopaedics, Changzheng Hospital, Shanghai, 200003, P.R.China.
Department of Orthopaedics, Changzheng Hospital, Shanghai, 200003,
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Jan 15;32(1):75-79. doi: 10.7507/1002-1892.201708126.
OBJECTIVE
To observe the structural changes of urinary center and the expression of Bcl-2 after conus medullaris injury in rats brain so as to explore the possible influence factors of degeneration in brain.
METHODS
Thirty-six adult Sprague-Dawley rats were randomly divided into experimental group ( =30) and control group ( =6). In the experimental group, the conus medullaris injury model was established by cutting off the spinal nerve below L , and no treatment was done in the control group. The modeling operations in the experimental group were successful, and 2 rats died at 3 months and 5 months after modeling operation respectively, which may be caused by renal failure or urinary tract infection. In the experimental group, 6, 6, 6, 5, and 5 rats were killed at 1 day, 1 week, and 1, 3, 6 months after operation respectively, and 1 rat was killed at each time point in the control group. The dorsolateral tissue of the pontine tegmentum was harvested to perform HE staining and Bcl-2 immunohistochemical SP staining.
RESULTS
HE staining showed that there was no obvious difference between the experimental group and the control group at 1 day after operation, the neurons were densely packed, arranged neatly, and the nucleoli were clear; at 1 week, the space between the neurons in the experimental group were slightly widened; at 1 month, nucleus retraction in some neurons happened in the experimental group; at 3 and 6 months, the nuclei in the experimental group were more and more condensed, and even some cells disappeared. Bcl-2 immunohistochemical SP staining showed that the expression of Bcl-2 in the control group was weakly positive. The positive expression of Bcl-2 was found at 1 day after operation in the experimental group; the positive expression of Bcl-2 at 7 days after operation was significantly higher than that in the control group, and reached the peak; the positive expression of Bcl-2 decreased gradually at 1, 3, and 6 months after modeling operation, but it was still higher than that of the control group.
CONCLUSION
The urinary center appears structure degeneration and necrocytosis after conus medullaris injury in rats brain. The elevated expression of Bcl-2 may be associated with brain tissue repair and function remodeling.
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