Ali Habib, Muhammad Abrar, Hou Youming
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.
Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.
J Microbiol Biotechnol. 2018 May 28;28(5):796-808. doi: 10.4014/jmb.1712.12019.
The intracellular bacterium is widespread in arthropods. Recently, possibilities of novel -mediated hosts, their distribution, and natural rate have been anticipated, and the coconut leaf beetle (Gestro) (Coleoptera: Chrysomelidae), which has garnered attention as a serious pest of palms, was subjected to this interrogation. By adopting surface protein () and multilocus sequence type (MLST) genotypic systems, we determined the infection density within host developmental stages, body parts, and tissues, and the results revealed that all the tested samples of were infected with the same strain (wLog), suggesting complete vertical transmission. The MLST profile elucidated two new alleles (-234 and coxA-266) that define a new sequence type (ST-483), which indicates the particular genotypic association of and . The quantitative real-time PCR analysis revealed a higher infection density in the eggs and adult stage, followed by the abdomen and reproductive tissues, respectively. However, no significant differences were observed in the infection density between sexes. Moreover, the and concatenated MLST alignment analysis of this study with other known Wolbachia-mediated arthropods revealed similar clustering with distinct monophyletic supergroup B. This is the first comprehensive report on the prevalence, infection dynamics, and phylogeny of the endosymbiont in , which demonstrated that is ubiquitous across all developmental stages and distributed in the entire body of . Understanding the infection dynamics would provide useful insight to build a framework for future investigations, understand its impacts on host physiology, and exploit it as a potential biocontrol agent.
这种细胞内细菌在节肢动物中广泛存在。最近，人们已经预见到了新型介导宿主的可能性、它们的分布以及自然发生率，并且对作为棕榈树严重害虫而备受关注的椰心叶甲（Gestro）（鞘翅目：叶甲科）进行了此项研究。通过采用表面蛋白（）和多位点序列分型（MLST）基因型系统，我们确定了宿主发育阶段、身体部位和组织内的感染密度，结果显示所有测试的椰心叶甲样本都感染了同一菌株（wLog），这表明存在完全垂直传播。MLST图谱阐明了两个新等位基因（-234和coxA-266），它们定义了一种新的序列类型（ST-483），这表明了与椰心叶甲的特定基因型关联。定量实时PCR分析显示，卵和成虫阶段的感染密度较高，其次分别是腹部和生殖组织。然而，两性之间的感染密度没有观察到显著差异。此外，本研究对椰心叶甲与其他已知的沃尔巴克氏体介导的节肢动物进行的和串联MLST比对分析显示，与不同的单系超群B有相似的聚类。这是关于椰心叶甲内共生菌的流行情况、感染动态和系统发育的第一份综合报告，该报告表明在椰心叶甲的所有发育阶段都普遍存在，并且分布在其整个身体中。了解感染动态将为构建未来研究的框架、理解其对宿主生理学的影响以及将其作为潜在的生物防治剂加以利用提供有用的见解。
