Division for Marine and Environmental Research, Ruder Bošković Institute, Zagreb, Croatia.
Unidad de Gestión Clínica de Aparato Digestivo, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain.
Surg Obes Relat Dis. 2018 Aug;14(8):1182-1191. doi: 10.1016/j.soard.2018.04.010. Epub 2018 Apr 24.
The changes that are produced in the gene expression of subcutaneous adipose tissue after Roux-en-Y gastric bypass are not yet fully known.
To identify the changes in the subcutaneous adipose tissue gene expression of morbidly obese women with low insulin resistance (MO-low-IR) and high insulin resistance (MO-high-IR) to find a relationship with measured obesity-related co-morbidities.
A university hospital.
Subcutaneous adipose tissue samples were assessed by microarray analysis before and 2 years after Roux-en-Y gastric bypass in MO-low-IR and MO-high-IR patients.
There is a group of shared differentially expressed genes (DEG) in both MO-low-IR and MO-high-IR, also there is a group of exclusive DEG in MO-low-IR and another group in MO-high-IR. In MO-high-IR, the downexpressed DEG are related to the regulation of transcription and are involved in the pathways related to cytokine-cytokine receptor interaction, cancer, phosphatidylinositol 3-kinase-protein kinase B signaling, human T-lymphotropic virus I infection, chemokine signaling, and Janus kinase/signal transducers and activators of transcription signaling. In MO-low-IR, the overexpressed DEG are related to carbohydrate metabolic processes, the downexpressed DEG to the glycosaminoglycan metabolic process and regulation of translation, and the pathways are related to phosphatidylinositol 3-kinase-protein kinase B signaling and metabolic pathways. The fold change of DEG mainly correlates with the percentage of change (Δ) of waist, Δhip, Δglucose, and Δtriglycerides. These DEG were mainly related to cancer, inflammation/immune regulation, metabolic pathways, ribonucleic acid/deoxyribonucleic acid regulation, virus infection, and regulation of cellular proliferation.
This study suggests a potential association between high insulin resistance and the expression of genes related to cancer and chronic immune activation/inflammation.
罗伊恩-Y 胃旁路术后皮下脂肪组织基因表达的变化尚不完全清楚。
鉴定胰岛素抵抗低（MO-low-IR）和胰岛素抵抗高（MO-high-IR）的病态肥胖女性皮下脂肪组织基因表达的变化，以找到与肥胖相关合并症的关系。
大学医院。
对 MO-low-IR 和 MO-high-IR 患者在罗伊恩-Y 胃旁路术前和术后 2 年的皮下脂肪组织样本进行微阵列分析。
在 MO-low-IR 和 MO-high-IR 中都存在一组共同差异表达基因（DEG），也存在一组 MO-low-IR 中特有的 DEG 和另一组 MO-high-IR 中的 DEG。在 MO-high-IR 中，下调的 DEG 与转录调控有关，与细胞因子-细胞因子受体相互作用、癌症、磷脂酰肌醇 3-激酶-蛋白激酶 B 信号转导、人类 T 淋巴细胞病毒 I 感染、趋化因子信号转导和 Janus 激酶/信号转导和转录激活物信号转导途径有关。在 MO-low-IR 中，过表达的 DEG 与碳水化合物代谢过程有关，下调的 DEG 与糖胺聚糖代谢过程和翻译调控有关，途径与磷脂酰肌醇 3-激酶-蛋白激酶 B 信号转导和代谢途径有关。DEG 的折叠变化主要与腰围、臀围、血糖和甘油三酯的变化百分比（Δ）相关。这些 DEG 主要与癌症、炎症/免疫调节、代谢途径、核糖核酸/脱氧核糖核酸调节、病毒感染和细胞增殖调节有关。
本研究提示高胰岛素抵抗与与癌症和慢性免疫激活/炎症相关的基因表达之间存在潜在关联。
