The Department of Pediatrics, The University Hospital Lübeck, Lübeck, Germany.
The Institute of Systemic Inflammation Research, The University Hospital Lübeck, Lübeck, Germany.
Pediatr Res. 2018 Jul;84(1):134-138. doi: 10.1038/s41390-018-0017-9. Epub 2018 May 28.
The aim of this study was to explore the role of the lectin pathway in neonatal sepsis through the study of MBL and MASP2 levels and their relationship with infection in a cohort of very-low-birth-weight infants (VLBWI).
MBL and MASP2 were measured in plasma samples of n = 89 VLBWI using ELISA and correlated with clinical parameters. MBL plasma levels were aligned with genotyping data of mbl2 exon 1 polymorphisms, rs1800450, rs1800451, and rs5030737.
MBL levels were clearly determined by MBL genotype, i.e., AA individuals had tenfold higher MBL levels than AO individuals. MBL and MASP2 levels did not correlate with gestational age, apart from MASP2 levels on day 7. During the first 21 days of life, we noted a gradual increase in both MBL and MASP2 levels. On day 7 of life, MASP2 levels in infants developing late-onset sepsis measured before the onset of symptoms were found to be lower, as compared to non-LOS infants.
In our cohort of VLBWI, MBL levels were genetically determined, but not associated with gestational age or sepsis in the first 21 days of life. Lower MASP2 levels on day 7 may indicate increased risk for late-onset infection.
本研究旨在通过研究 MBL 和 MASP2 水平及其与感染的关系，探讨凝集素途径在新生儿败血症中的作用。
采用 ELISA 法检测 89 例极低出生体重儿（VLBWI）血浆 MBL 和 MASP2 水平，并与临床参数相关分析。MBL 血浆水平与 mbl2 外显子 1 多态性 rs1800450、rs1800451 和 rs5030737 的基因分型数据进行了关联分析。
MBL 水平明显受 MBL 基因型决定，即 AA 个体的 MBL 水平比 AO 个体高 10 倍。MBL 和 MASP2 水平与胎龄无关，除第 7 天的 MASP2 水平外。在生命的前 21 天，我们观察到 MBL 和 MASP2 水平逐渐增加。在发病前测定的第 7 天发生晚发性败血症的婴儿的 MASP2 水平低于非 LOS 婴儿。
在我们的 VLBWI 队列中，MBL 水平由遗传决定，但与胎龄或前 21 天的败血症无关。第 7 天 MASP2 水平较低可能表明晚发性感染的风险增加。
