Elolimy Ahmed A, Moisá Sonia J, Brennan Kristen M, Smith Allison C, Graugnard Daniel, Shike Daniel W, Loor Juan J
Mammalian NutriPhysioGenomics, Department of Animal Sciences, University of Illinois, Urbana, Illinois.
Department of Animal Sciences, University of Illinois, Urbana, Illinois.
Anim Sci J. 2018 Aug;89(8):1107-1119. doi: 10.1111/asj.13041. Epub 2018 May 29.
Our main objective was to evaluate the effects of feeding α-amylase (Amaize, Alltech Inc., Nicholasville, KY, USA) for 140 days on skeletal muscle and liver gene transcription in beef steers. Steers fed Amaize had lower average daily gain (p = .03) and gain:feed ratio (p = .05). No differences (p > .10) in serum metabolites or carcass traits were detected between the two groups but Amaize steers tended (p < .15) to have increased 12th rib fat depth. Microarray analysis of skeletal muscle revealed 21 differentially expressed genes (DEG), where 14 were up-regulated and seven were down-regulated in Amaize-fed steers. The bioinformatics analysis indicated that metabolic pathways involved in fat formation and deposition, stress response, and muscle function were activated, while myogenesis was inhibited in Amaize-fed steers. The quantitative PCR results for liver revealed a decrease (p < .01) in expression of fatty acid binding protein 1 (FABP1) and 3-hydroxybutyrate dehydrogenase 1 (BDH1) with Amaize. Because these genes are key for intracellular fatty acid transport, oxidation and ketone body production, data suggest a reduction in hepatic lipid catabolism. Future work to investigate potential positive effects of Amaize on cellular stress response, muscle function, and liver function in beef cattle appears warranted.
我们的主要目标是评估在140天内给肉牛饲喂α-淀粉酶（Amaize，美国肯塔基州尼古拉斯维尔市奥特奇公司）对其骨骼肌和肝脏基因转录的影响。饲喂Amaize的肉牛平均日增重较低（p = 0.03），增重与饲料比也较低（p = 0.05）。两组之间血清代谢物或胴体性状没有差异（p > 0.10），但饲喂Amaize的肉牛第12肋处的脂肪厚度有增加的趋势（p < 0.15）。骨骼肌的微阵列分析显示有21个差异表达基因（DEG），在饲喂Amaize的肉牛中，14个基因上调，7个基因下调。生物信息学分析表明，参与脂肪形成和沉积、应激反应及肌肉功能的代谢途径被激活，而在饲喂Amaize的肉牛中，肌生成受到抑制。肝脏的定量PCR结果显示，饲喂Amaize时，脂肪酸结合蛋白1（FABP1）和3-羟基丁酸脱氢酶1（BDH1）的表达下降（p < 0.01）。由于这些基因是细胞内脂肪酸转运、氧化和酮体生成的关键，数据表明肝脏脂质分解代谢减少。未来有必要开展研究，以探究Amaize对肉牛细胞应激反应、肌肉功能和肝功能的潜在积极影响。
