Hatab Shaimaa, Lin Kaihe, Miao Wenhua, Chen Meiling, Lin Jiheng, Deng Shanggui
1 College of Food Science and Pharmaceutics, Zhejiang Ocean University , Zhoushan, China .
2 Faculty of Environmental Agricultural Science, Arish University , North Sinai, Egypt .
Foodborne Pathog Dis. 2018 Aug;15(8):498-505. doi: 10.1089/fpd.2017.2422. Epub 2018 May 29.
Ethanolic extracts of green tea leaves (GTE) and fenugreek seeds (FSE) were screened for their antibacterial activity against four food pathogenic strains using disc diffusion method. The two extracts revealed antimicrobial activity against selected bacterial strains. GTE showed the highest antibacterial activity to Escherichia coli and Staphylococcus aureus at a concentration of 1% with inhibition zone equal to 29.45 ± 0.64 mm and 25.68 ± 1.2 mm, respectively. In addition, the effect of GTE and FSE combined with chitosan coating on the shelf life of Pacific white shrimp (PWS) (Litopenaeus vannamei) during refrigerated storage have been studied. Our results indicated that using GTE or FSE during the refrigerated storage of PWS led to significantly decreased Total Volatile Bases Nitrogen, Thiobarbituric acid reacting substances, total bacterial count, and pH. The sensory properties of PWS have improved considerably in the samples treated GTE or FSE. These findings suggested that the application of chitosan coating combined with GTE or FSE to PWS is advisable to achieve better quality during refrigerated storage.
采用纸片扩散法，对绿茶叶乙醇提取物（GTE）和胡芦巴籽乙醇提取物（FSE）针对四种食源性病原体菌株的抗菌活性进行了筛选。这两种提取物对选定的细菌菌株均显示出抗菌活性。GTE在浓度为1%时，对大肠杆菌和金黄色葡萄球菌表现出最高的抗菌活性，抑菌圈分别为29.45±0.64毫米和25.68±1.2毫米。此外，还研究了GTE和FSE与壳聚糖涂层相结合对冷藏期间南美白对虾（PWS）（凡纳滨对虾）货架期的影响。我们的结果表明，在PWS冷藏储存期间使用GTE或FSE可显著降低总挥发性盐基氮、硫代巴比妥酸反应物、总细菌数和pH值。经GTE或FSE处理的PWS样品的感官特性有了相当大的改善。这些发现表明，将壳聚糖涂层与GTE或FSE结合应用于PWS，有利于在冷藏储存期间获得更好的品质。
