Carr B, Johnson G
Basic Life Sci. 1979;13:521-8. doi: 10.1007/978-1-4613-3069-1_27.
Investigations of polyploidy using electrophoresis are at present severely limited by several areas of difficulty which limit all applications of this technique. The technical problems inherent in electrophoresis of plants, namely extraction of active extracts and maximizing resolution of electrophoretic variants through investigation of gel and especially assay conditions, have never been addressed explicitly. No rationale for initiating work on a new species is available. Analytical approaches to defining the conditions which limit resolution are rare. Gel-to-gel variation is poorly controlled, seldom monitored. The individual nature of electrophoretic investigations limits the comparability of data among labs. In short, "hit-and-miss" approaches predominate, and these limit investigation. Techniques are now available which one can hope will greatly improve the experimental situation.
目前，利用电泳技术对多倍体进行的研究受到若干困难领域的严重限制，这些困难限制了该技术的所有应用。植物电泳中固有的技术问题，即活性提取物的提取以及通过研究凝胶，尤其是分析条件来最大化电泳变体的分辨率，从未得到明确解决。没有启动对新物种研究工作的理论依据。界定限制分辨率条件的分析方法很少见。凝胶间的差异控制不佳，很少进行监测。电泳研究的个体性质限制了不同实验室之间数据的可比性。简而言之，“试错”方法占主导，这些方法限制了研究。现在有一些技术有望极大地改善实验状况。
