Leb L, Beatson P, Fortier N, Newburger P E, Snyder L M
J Leukoc Biol. 1985 Apr;37(4):449-59. doi: 10.1002/jlb.37.4.449.
The in vitro effect of colloidal suspensions of alpha-tocopherol (alpha-T) on phorbolmyristate-acetate (PMA)-induced monocyte cytotoxicity and on antibody-dependent monocyte cytotoxicity (ADCC) was studied. We observed that 1) in the presence of alpha-T, the inhibition was twice as high in the PMA-induced assay than in ADCC; 2) monocytes preincubated with alpha-T were inhibitory in both assays but much less in ADCC, and 3) target erythrocytes preincubated with alpha-T decreased the cytotoxicity in the PMA-induced assay only. Since alpha-T preincubated monocytes showed a decreased release of H2O2 but not of O2-, we concluded that one of the mechanisms by which alpha-T decreased cytotoxicity could be decreased release of H2O2. Whereas the role of H2O2 was documented in the PMA-induced cytotoxicity, in ADCC non-oxidative injury seems more important. This is supported by 1) lesser inhibition of the assay with alpha-T preincubated monocytes; 2) lack of protection with alpha-T preincubated erythrocytes, and 3) mild inhibition with protease inhibitor.
研究了α-生育酚（α-T）胶体悬浮液对佛波酯肉豆蔻酸酯乙酸酯（PMA）诱导的单核细胞细胞毒性以及对抗体依赖性单核细胞细胞毒性（ADCC）的体外作用。我们观察到：1）在α-T存在的情况下，PMA诱导试验中的抑制作用比ADCC试验中的抑制作用高两倍；2）用α-T预孵育的单核细胞在两种试验中均具有抑制作用，但在ADCC试验中的抑制作用要小得多，并且3）用α-T预孵育的靶红细胞仅在PMA诱导试验中降低了细胞毒性。由于用α-T预孵育的单核细胞显示H2O2的释放减少，但O2-的释放未减少，因此我们得出结论，α-T降低细胞毒性的机制之一可能是H2O2的释放减少。虽然H2O2在PMA诱导的细胞毒性中的作用已有文献记载，但在ADCC中，非氧化损伤似乎更为重要。这得到以下几点的支持：1）用α-T预孵育的单核细胞对试验的抑制作用较小；2）用α-T预孵育的红细胞缺乏保护作用，以及3）蛋白酶抑制剂的抑制作用较弱。
