佛波酯对淋巴细胞中Na+/H+交换激活的特性：反向转运体胞质pH依赖性的变化
Characterization of the activation of Na+/H+ exchange in lymphocytes by phorbol esters: change in cytoplasmic pH dependence of the antiport.
作者信息
Grinstein S, Cohen S, Goetz J D, Rothstein A, Gelfand E W
出版信息
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1429-33. doi: 10.1073/pnas.82.5.1429.
Abstract
Phorbol 12-myristate 13-acetate and other phorbol esters induce an intracellular alkalinization in rat thymic lymphocytes. An extracellular acidification can be recorded concomitantly. This transmembrane H+ (equivalent) flux is dependent on external Na+ and is amiloride sensitive. Phorbol esters also stimulate an amiloride-sensitive uptake of 22Na+, suggesting activation of Na+o/H+i exchange. Only those phorbol derivatives that are tumor promoters and activators of protein kinase C stimulate the antiport. Activation of the Na+/H+ exchange is brought about by a change in the cytoplasmic pH sensitivity of the antiport. Activation of the Na+/H+ exchanger by phorbol esters results in membrane hyperpolarization, due to indirect stimulation of the electrogenic Na+/K+ pump by the increased intracellular Na+ concentration. Increased Na+/H+ exchange also produces cell swelling, which may be one of the earliest manifestations of the growth-promoting properties of the phorbol esters.
摘要
佛波醇12 -肉豆蔻酸酯13 -乙酸酯及其他佛波酯可诱导大鼠胸腺淋巴细胞发生细胞内碱化。同时可记录到细胞外酸化。这种跨膜H⁺（当量）通量依赖于细胞外Na⁺且对氨氯地平敏感。佛波酯还能刺激氨氯地平敏感的²²Na⁺摄取，提示Na⁺o/H⁺i交换被激活。只有那些作为肿瘤促进剂和蛋白激酶C激活剂的佛波衍生物能刺激这种反向转运体。Na⁺/H⁺交换的激活是由反向转运体对细胞质pH敏感性的改变引起的。佛波酯激活Na⁺/H⁺交换器会导致膜超极化，这是由于细胞内Na⁺浓度增加间接刺激了生电Na⁺/K⁺泵。增加的Na⁺/H⁺交换也会导致细胞肿胀，这可能是佛波酯促生长特性的最早表现之一。
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