Robain O, Lanfumey L, Adrien J, Farkas E
Exp Neurol. 1985 Apr;88(1):150-64. doi: 10.1016/0014-4886(85)90120-7.
Three-day-old rats received an infusion of a small dose of 6-hydroxydopamine (6-OHDA) (12 micrograms) directly into the locus ceruleus nuclei, in order to induce a subtotal lesion of the noradrenergic system. Significant modifications in cerebellum maturation were observed, mainly a regression failure in the perisomatic Purkinje cell processes in the depth of the fissura prima of 10-day-old rats, a reduction in the whole vermis surface area and in the total number of internal granule cells at 15 days of age, the number of external granule cells was slightly enhanced. To discriminate between the effects of the noradrenergic deafferentation and the nonspecific toxic effects of 6-OHDA, these results were compared to those obtained after an infusion of 6-OHDA + bovine serum albumin (BSA) into the locus ceruleus (which produced a selective deafferentation) and after an injection of 6-OHDA directly into the IVth ventricle (which increased the nonspecific toxic effects). Whatever the procedure used, the same reduction in the size of the vermis was observed. However, the fissuration index was not modified when BSA was added to the 6-OHDA solution. We conclude that noradrenergic deafferentation by itself was sufficient to reduce the growth of the cerebellum at certain periods of postnatal life.
对出生三天的大鼠，将小剂量（12微克）的6-羟基多巴胺（6-OHDA）直接注入蓝斑核，以诱导去甲肾上腺素能系统的部分损伤。观察到小脑成熟有显著改变，主要表现为10日龄大鼠在原裂深处的躯体周围浦肯野细胞突起的退化失败，15日龄时整个蚓部表面积和内部颗粒细胞总数减少，而外部颗粒细胞数量略有增加。为了区分去甲肾上腺素能脱失的影响和6-OHDA的非特异性毒性作用，将这些结果与向蓝斑核注入6-OHDA + 牛血清白蛋白（BSA）（产生选择性脱失）以及将6-OHDA直接注入第四脑室（增加非特异性毒性作用）后获得的结果进行比较。无论采用何种方法，都观察到蚓部大小有相同程度的减小。然而，当在6-OHDA溶液中加入BSA时，裂沟指数未发生改变。我们得出结论，去甲肾上腺素能脱失本身足以在出生后生命的某些时期减少小脑的生长。
