Coukell M B, Cameron A M
J Bacteriol. 1985 Apr;162(1):427-9. doi: 10.1128/jb.162.1.427-429.1985.
Previous attempts to map the stmF locus in Dictyostelium discoideum, by using only clone morphology as a marker, have led to equivocal results. Since strains carrying mutations at the stmF locus possess very low cyclic GMP phosphodiesterase activity, we have remapped this locus using both morphological and biochemical markers. Our results indicate that mutations producing a stable "streamer" phenotype and reduced cyclic GMP phosphodiesterase activity are located in linkage group II, probably centromere distal to acrA.
以前仅通过克隆形态作为标记来定位盘基网柄菌中stmF基因座的尝试,得到了不明确的结果。由于在stmF基因座携带突变的菌株具有非常低的环鸟苷酸磷酸二酯酶活性,我们使用形态学和生化标记重新定位了该基因座。我们的结果表明,产生稳定的“拖尾”表型和降低的环鸟苷酸磷酸二酯酶活性的突变位于连锁群II中,可能在acrA的着丝粒远端。