Kalyanaraman B, Janzen E G, Mason R P
J Biol Chem. 1985 Apr 10;260(7):4003-6.
The azidyl radical is formed during the oxidation of sodium azide by the catalase/hydrogen peroxide system, as detected by the ESR spin-trapping technique. The oxidation of azide by horseradish peroxidase, chloroperoxidase, lactoperoxidase, and myeloperoxidase also forms azidyl radical. It is suggested that the evolution of nitrogen gas and nitrogen oxides reported in the azide/catalase/hydrogen peroxide system results from reactions of the azidyl radical. The azide/horseradish peroxidase/hydrogen peroxide system consumes oxygen, and this oxygen uptake is inhibited by the spin trap 5,5-dimethyl-1-pyrroline-N-oxide, presumably due to the competition with oxygen for the azidyl radical. Although azide is used routinely as an inhibitor of myeloperoxidase and catalase, some consideration should be given to the biochemical consequences of the formation of the highly reactive azidyl radical by the peroxidase activity of these enzymes.
通过电子自旋共振（ESR）自旋捕集技术检测发现，在过氧化氢酶/过氧化氢体系氧化叠氮化钠的过程中会形成叠氮基自由基。辣根过氧化物酶、氯过氧化物酶、乳过氧化物酶和髓过氧化物酶氧化叠氮化物时也会形成叠氮基自由基。有人认为，叠氮化物/过氧化氢酶/过氧化氢体系中报道的氮气和氮氧化物的释放是由叠氮基自由基的反应引起的。叠氮化物/辣根过氧化物酶/过氧化氢体系消耗氧气，这种氧气摄取受到自旋捕集剂5,5-二甲基-1-吡咯啉-N-氧化物的抑制，这可能是由于其与氧气竞争叠氮基自由基所致。尽管叠氮化物通常用作髓过氧化物酶和过氧化氢酶的抑制剂，但应考虑这些酶的过氧化物酶活性形成高反应性叠氮基自由基所带来的生化后果。
