Mottley C, Robinson R E, Mason R P
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Arch Biochem Biophys. 1991 Aug 15;289(1):153-60. doi: 10.1016/0003-9861(91)90455-r.
Malondialdehyde, a product of lipid peroxidation, and acetylacetone undergo one-electron oxidation by peroxidase enzymes to form free radical metabolites, which were detected with ESR using the spin-trapping technique. The structures of the radical adducts were assigned using isotope substitution. With both malondialdehyde and acetylacetone and the enzymes myeloperoxidase and chloroperoxidase, carbon-centered radicals were detected. With horseradish peroxidase, a carbon-centered radical was initially trapped and then disappeared with the concomitant appearance of an iminoxyl radical.
丙二醛是脂质过氧化产物，它与乙酰丙酮通过过氧化物酶进行单电子氧化，形成自由基代谢产物，这些产物通过自旋捕获技术用电子自旋共振（ESR）进行检测。自由基加合物的结构通过同位素取代来确定。使用丙二醛、乙酰丙酮以及髓过氧化物酶和氯过氧化物酶时，检测到了以碳为中心的自由基。使用辣根过氧化物酶时，最初捕获到一个以碳为中心的自由基，随后该自由基消失，同时出现一个亚胺氧基自由基。
