Maruyama I, Salem H H, Ishii H, Majerus P W
J Clin Invest. 1985 Mar;75(3):987-91. doi: 10.1172/JCI111800.
The effect of human thrombomodulin isolated from placenta on the procoagulant activity of thrombin was studied and compared to that of rabbit thrombomodulin. The isolated protein was proved to be thrombomodulin because a rabbit antibody against the isolated protein blocked protein C activation by thrombomodulin in solution and also blocked the protein-C-activating cofactor activity of human umbilical vein endothelial cells. The affinity of human thrombomodulin for human thrombin in the presence of fibrinogen is 30 times less than that of rabbit thrombomodulin. This value is based on the measurements of the clotting time of human fibrinogen and thrombin in the presence of increasing amounts of thrombomodulin. Human thrombomodulin was also much less effective compared with rabbit thrombomodulin in inhibiting thrombin-induced human coagulation factor V activation. The ability to inhibit release of [3H]serotonin from washed human platelets was at least 10 times less using human thrombomodulin compared with rabbit thrombomodulin. A partially purified preparation of human lung thrombomodulin was also relatively ineffective in inhibiting thrombin-induced serotonin release from platelets, indicating that the difference between rabbit and human thrombomodulin is one of species rather than of tissue. Thus, while human thrombomodulin is a potent cofactor in protein C activation, it is not an efficient inhibitor of the procoagulant actions of thrombin.
研究了从胎盘中分离出的人血栓调节蛋白对凝血酶促凝活性的影响，并与兔血栓调节蛋白进行了比较。所分离的蛋白质被证明是血栓调节蛋白，因为针对该分离蛋白的兔抗体可抑制溶液中血栓调节蛋白对蛋白C的激活，还可阻断人脐静脉内皮细胞的蛋白C激活辅因子活性。在存在纤维蛋白原的情况下，人血栓调节蛋白对人凝血酶的亲和力比兔血栓调节蛋白低30倍。该数值基于在存在不同量血栓调节蛋白的情况下对人纤维蛋白原和凝血酶凝血时间的测量。与人血栓调节蛋白相比，兔血栓调节蛋白在抑制凝血酶诱导的人凝血因子V激活方面也更有效。与人血栓调节蛋白相比，使用兔血栓调节蛋白抑制洗涤后的人血小板释放[3H]5-羟色胺的能力至少低10倍。人肺血栓调节蛋白的部分纯化制剂在抑制凝血酶诱导的血小板5-羟色胺释放方面也相对无效，这表明兔和人血栓调节蛋白之间的差异是物种差异而非组织差异。因此，虽然人血栓调节蛋白是蛋白C激活的有效辅因子，但它不是凝血酶促凝作用的有效抑制剂。
