Kreisle R A, Parker C W, Griffin G L, Senior R M, Stenson W F
J Immunol. 1985 May;134(5):3356-63.
Rat PMN isolated from peripheral blood show a small amount of high-affinity (specific) binding of [3H]-LTB4 at nanomolar concentrations. This binding is reversible and has a stereospecificity similar to rat PMN aggregation in response to several LTB4 analogs. This population of binding sites shares many characteristics with a population of high-affinity binding sites in human PMN; however, human PMN bind a significantly greater amount of [3H]-LTB4 to a second population of specific binding sites that is not present in rat PMN. The aggregation responses of human and rat peripheral blood PMN to LTB4 are similar in magnitude and specificity, but unlike human PMN, LTB4 fails to elicit a chemotactic response in rat PMN at concentrations from 10(-10) M to 10(-6) M. Rat PMN also fail to metabolize exogenous LTB4 when compared with human PMN. These data suggest that different PMN functions, such as chemotaxis and aggregation, may involve different classes of specific receptors. The finding that rat PMN do not exhibit chemotaxis to LTB4 calls for a reevaluation of the relevance to inflammation in humans of studies of inflammation performed in rat models.
从外周血分离的大鼠中性粒细胞在纳摩尔浓度下显示出少量[3H]-白三烯B4的高亲和力（特异性）结合。这种结合是可逆的，并且具有与大鼠中性粒细胞对几种白三烯B4类似物的聚集反应相似的立体特异性。这群结合位点与人类中性粒细胞中的一群高亲和力结合位点具有许多共同特征；然而，人类中性粒细胞与大鼠中性粒细胞中不存在的第二群特异性结合位点结合了大量的[3H]-白三烯B4。人类和大鼠外周血中性粒细胞对白三烯B4的聚集反应在程度和特异性上相似，但与人类中性粒细胞不同，白三烯B4在10(-10)M至10(-6)M的浓度下未能在大鼠中性粒细胞中引发趋化反应。与人类中性粒细胞相比，大鼠中性粒细胞也不能代谢外源性白三烯B4。这些数据表明，不同的中性粒细胞功能，如趋化性和聚集，可能涉及不同类别的特异性受体。大鼠中性粒细胞对白三烯B4不表现出趋化性这一发现，需要重新评估在大鼠模型中进行的炎症研究与人类炎症的相关性。
