CD8阳性而非CD56阳性的CD8细胞主要表达杀伤细胞免疫球蛋白样受体（KIR），并且在内脏利什曼病期间具有细胞毒性。
CD8 but not CD8 cells positive to CD56 dominantly express KIR and are cytotoxic during visceral leishmaniasis.
作者信息
Kumari Sarita, Shivam Pushkar, Hansa Jagadish, Jamal Fauzia, Singh Manish Kumar, Bimal Sanjiva, Narayan Shyam, Pandey Krishna, Das Vidya Nand Ravi, Das Pradeep, Singh Shubhankar K
机构信息
Indian Council of Medical Research-Rajendra Memorial Research Institute of Medical Sciences (ICMR-RMRIMS), Agamkuan, Patna 800007, India.
Indian Council of Medical Research-Rajendra Memorial Research Institute of Medical Sciences (ICMR-RMRIMS), Agamkuan, Patna 800007, India.
出版信息
Hum Immunol. 2018 Aug;79(8):616-620. doi: 10.1016/j.humimm.2018.05.004. Epub 2018 May 26.
This study reports a structural and functional heterogeneity of CD8CD56NKT cells, which usually decrease quantitatively during visceral leishmaniasis. Based on fluorescence intensity of CD8 receptors on CD56NKT cells, two populations of CD8CD56NKT cells have been identified. These cells were recognized as CD8CD56NKT and CD8CD56NKT cells. We further analyzed the functional nature of CD8 and CD8 positive CD56NKT cells. In comparison to CD8CD56NKT cells, a significantly higher percentage of CD8CD56NKT cells expressed KIR during VL. The percentage of CD8CD56NKT cells expressing KIR was found 4 fold higher in VL as compared to healthy subjects. But, the difference was insignificant in case of CD8CD56NKT cells. CD8CD56NKT cells release granzyme B to kill the infected cells. A categorical difference was also observed in the function of CD8CD56NKT and CD8CD56NKT cells during visceral leishmaniasis. The percentage of granzyme B expressing CD8CD56NKT cells was 2.83 fold higher in VL compared to healthy subjects. But, there was no significant difference in granzyme B expressing CD8CD56NKT cells in samples from healthy and VL subjects. However, within VL subject, the percentage of granzyme B expressing CD8CD56NKT cells was 5.7 fold higher in comparison to CD8CD56NKT cells. This study concludes that CD8CD56NKT cells are more cytotoxic than CD8CD56NKT cells during VL.
本研究报告了CD8CD56 NKT细胞的结构和功能异质性，这类细胞在内脏利什曼病期间通常会在数量上减少。基于CD56 NKT细胞上CD8受体的荧光强度，已鉴定出两类CD8CD56 NKT细胞。这些细胞分别被识别为CD8⁺CD56 NKT和CD8⁻CD56 NKT细胞。我们进一步分析了CD8⁺和CD8⁻阳性CD56 NKT细胞的功能特性。与CD8⁻CD56 NKT细胞相比，在VL期间，CD8⁺CD56 NKT细胞表达KIR的比例显著更高。与健康受试者相比，VL患者中表达KIR的CD8⁺CD56 NKT细胞比例高出4倍。但是，CD8⁻CD56 NKT细胞的情况差异不显著。CD8⁺CD56 NKT细胞释放颗粒酶B以杀死被感染的细胞。在内脏利什曼病期间，CD8⁺CD56 NKT和CD8⁻CD56 NKT细胞的功能也存在显著差异。与健康受试者相比，VL患者中表达颗粒酶B的CD8⁺CD56 NKT细胞比例高出2.83倍。但是，健康受试者和VL患者样本中表达颗粒酶B的CD8⁻CD56 NKT细胞没有显著差异。然而，在VL患者中，表达颗粒酶B的CD8⁺CD56 NKT细胞比例相较于CD8⁻CD56 NKT细胞高出5.7倍。本研究得出结论，在内脏利什曼病期间，CD8⁺CD56 NKT细胞比CD8⁻CD56 NKT细胞更具细胞毒性。
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