Kuus K, Ottenbrite R M, Kaplan A M
J Biol Response Mod. 1985 Feb;4(1):46-59.
Peritoneal macrophages (PM theta) from mice treated intraperitoneally with the unique polyanionic compounds cyclohexyl-1,3-dioxepin maleic anhydride copolymer (CDA-MA) and 4-methyl-2-pentenoyl maleic anhydride copolymer (MP-MA) had tumoricidal activity against Lewis lung tumor cells. 5'-Nucleotidase ectoenzyme activity, which had previously been associated only with nontumoricidal, resident PM theta, was elevated in tumoricidal PM theta elicited with CDA-MA and MP-MA. Cell counts and differentials performed on peripheral blood leukocytes and PM theta populations from CDA-MA- and MP-MA--treated mice more closely corresponded to those of normal mice than mice treated with the conventional PM theta-activating agents pyran and Corynebacterium parvum. In addition, the lysosomal peroxidase activity in PM theta after administration of CDA-MA and MP-MA remained at levels comparable with normal resident PM theta, while an influx of peroxidase-positive macrophages was observed after administration of pyran and C. parvum. Inoculation of CDA-MA and MP-MA into mice bearing Lewis lung carcinoma showed a significant increase in median survival time compared with control mice, as well as an increase in the percentage of mice that survived greater than 90 days. Taken together, these data suggest that CDA-MA and MP-MA activated PM theta in situ and prolonged survival against primary transplanted Lewis lung carcinoma.
用独特的聚阴离子化合物环己基-1,3-二氧杂环庚烷马来酸酐共聚物（CDA-MA）和4-甲基-2-戊烯酰马来酸酐共聚物（MP-MA）腹腔注射处理的小鼠的腹腔巨噬细胞（PMθ）对Lewis肺癌细胞具有杀肿瘤活性。以前仅与无杀肿瘤活性的驻留PMθ相关的5'-核苷酸酶外切酶活性，在由CDA-MA和MP-MA诱导的具有杀肿瘤活性的PMθ中升高。对来自用CDA-MA和MP-MA处理的小鼠的外周血白细胞和PMθ群体进行的细胞计数和分类，比用传统的PMθ激活剂吡喃和短小棒状杆菌处理的小鼠更接近正常小鼠。此外，给予CDA-MA和MP-MA后PMθ中的溶酶体过氧化物酶活性保持在与正常驻留PMθ相当的水平，而给予吡喃和短小棒状杆菌后观察到过氧化物酶阳性巨噬细胞的流入。将CDA-MA和MP-MA接种到患有Lewis肺癌的小鼠中，与对照小鼠相比，中位生存时间显著增加，并且存活超过90天的小鼠百分比也增加。综上所述，这些数据表明CDA-MA和MP-MA在原位激活了PMθ，并延长了对原发性移植Lewis肺癌的生存期。
