Biological Anthropology Institute, Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
Department of Cardiology, Jinzhou Central Hospital, Jinzhou, Liaoning 121000, P.R. China.
Int J Oncol. 2018 Aug;53(2):815-822. doi: 10.3892/ijo.2018.4415. Epub 2018 May 22.
Cluster of ddifferentiation 24 (CD24) is a widely used cancer stem cell (CSC) marker in numerous cancer types. However, a number of studies have shown that CD24 is a prognostic marker, but not a CSC marker for lung adenocarcinoma. In the present study, firstly, bioinformatic analyses were used to identify the CD24 mRNA levels in the subtypes of lung cancer. Secondly, CD24high and CD24low cells were isolated from the side population of Lewis lung carcinoma (LLC) cells using flow cytometry. Furthermore, the stemness of CD24high and CD24low cells were determined in vivo and in vitro. Lastly, the mechanism(s) of nicotine-inhibited CD24 expression in LLC cells were assessed. The main findings of this study are that: i) CD24 could be used as a prognostic marker for human lung adenocarcinoma; ii) the in vitro and in vivo experiments did not determine a significant influence of CD24 on the tumorgenicity of LLC cells; and iii) nicotine inhibited CD24 expression in LLC cells by upregulation of RAS. However, the detailed mechanism(s) of these results require further analysis.
簇分化 24（CD24）是许多癌症类型中广泛使用的癌症干细胞（CSC）标志物。然而，许多研究表明，CD24 是一种预后标志物，但不是肺腺癌的 CSC 标志物。在本研究中，首先，使用生物信息学分析来鉴定肺癌亚型中的 CD24 mRNA 水平。其次，使用流式细胞术从 Lewis 肺癌（LLC）细胞的侧群中分离出 CD24high 和 CD24low 细胞。此外，在体内和体外确定 CD24high 和 CD24low 细胞的干性。最后，评估了尼古丁抑制 LLC 细胞中 CD24 表达的机制。本研究的主要发现是：i）CD24 可作为人类肺腺癌的预后标志物；ii）体外和体内实验并未确定 CD24 对 LLC 细胞致瘤性的显著影响；iii）尼古丁通过上调 RAS 抑制 LLC 细胞中 CD24 的表达。然而，这些结果的详细机制需要进一步分析。
