Danikowski Kevin M, Cheng Tong
Department of Biology, William Rainey Harper College, Palatine, IL, 60067, USA.
Curr Microbiol. 2018 Sep;75(9):1226-1230. doi: 10.1007/s00284-018-1514-0. Epub 2018 May 30.
Staphylococcus aureus is known for its resistance to antibiotic treatment as well as the ability to form biofilms. Biofilm formation has been seen in S. aureus infections, yet, the mechanism of biofilm formation is not completely understood. Many molecules, such as DNA and polysaccharides, have been identified in the biofilm microenvironment, but little is known about the enzymes involved in the process. In this paper, alkaline phosphatase (ALP) activity was investigated in S. aureus grown either in biofilm or suspension cultures, achieved using DNase I. A significant increase of ALP activity was observed in S. aureus biofilm culture compared to its suspension counterpart. Treatment of sodium orthovanadate, an ALP inhibitor, significantly decreased biofilm formation. Its inhibition was on par with DNase I treatment at specific doses. Thus, ALP may play an important role in the biofilm formation. Likewise, ALP inhibition may be a novel target for anti-biofilm therapeutics.
金黄色葡萄球菌以其对抗生素治疗的抗性以及形成生物膜的能力而闻名。在金黄色葡萄球菌感染中已观察到生物膜的形成，然而，生物膜形成的机制尚未完全了解。在生物膜微环境中已鉴定出许多分子，如DNA和多糖，但对该过程中涉及的酶了解甚少。在本文中，使用脱氧核糖核酸酶I研究了在生物膜或悬浮培养物中生长的金黄色葡萄球菌的碱性磷酸酶（ALP）活性。与悬浮培养物相比，在金黄色葡萄球菌生物膜培养物中观察到ALP活性显著增加。碱性磷酸酶抑制剂原钒酸钠的处理显著减少了生物膜的形成。在特定剂量下，其抑制作用与脱氧核糖核酸酶I处理相当。因此，碱性磷酸酶可能在生物膜形成中起重要作用。同样，碱性磷酸酶抑制可能是抗生物膜治疗的一个新靶点。
