Jack Brittany, Avasthi Prachee
Anatomy and Cell Biology Department, The University of Kansas Medical Center, Kansas City, KS, USA.
Department of Ophthalmology, The University of Kansas, Lawrence, KS, USA.
Methods Mol Biol. 2018;1795:203-221. doi: 10.1007/978-1-4939-7874-8_17.
Flagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of vertebrate cells and provide an excellent model to study ciliogenesis. Cilia and flagella are important organelles used for motility and sensing the extracellular environment. Abnormalities in cilia structure or ciliary dysfunction can have devastating consequences ranging from diabetes and obesity to polycystic kidney disease and mental retardation. Small-molecule inhibitor libraries can be used to screen for flagellum-associated phenotypes in assembly, length, motility, deflagellation, and cellular toxicity. These phenotypes can be assessed from direct microscopic visualization and custom-designed assays. These methods identify fundamental regulators of ciliary biology as well as potential therapeutic interventions for ciliopathies.
单细胞绿藻莱茵衣藻的鞭毛与脊椎动物细胞的纤毛几乎完全相同，是研究纤毛发生的理想模型。纤毛和鞭毛是用于运动和感知细胞外环境的重要细胞器。纤毛结构异常或纤毛功能障碍会导致从糖尿病、肥胖到多囊肾病和智力迟钝等一系列严重后果。小分子抑制剂文库可用于筛选鞭毛在组装、长度、运动、去鞭毛作用和细胞毒性方面的相关表型。这些表型可以通过直接显微镜观察和定制检测来评估。这些方法可识别纤毛生物学的基本调节因子以及纤毛病的潜在治疗干预措施。
