Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou 225001, China.
Jiangsu Key Laboratory of Experimental and Translational Noncoding RNA Research, Yangzhou 225001, China.
Asian J Androl. 2018 Sep-Oct;20(5):479-487. doi: 10.4103/aja.aja_26_18.
To clarify the functions and mechanism of stimulated by retinoic acid gene 8 (Stra8) in spermatogenesis, we analyzed the testes from Stra8 knockout and wild-type mice during the first wave of spermatogenesis. Comparisons showed no significant differences in morphology and number of germ cells at 11 days postpartum, while 21 differentially expressed genes (DEGs) associated with spermatogenesis were identified. We speculate that Stra8 performs many functions in different phases of spermatogenesis, such as establishment of spermatogonial stem cells, spermatogonial proliferation and self-renewal, spermatogonial differentiation and meiosis, through direct or indirect regulation of these DEGs. We therefore established a preliminary regulatory network of Stra8 during spermatogenesis. These results will provide a theoretical basis for further research on the mechanism underlying the role of Stra8 in spermatogenesis.
为了阐明视黄酸诱导基因 8（Stra8）在精子发生中的功能和机制，我们分析了 Stra8 敲除和野生型小鼠在第一次精子发生波期间的睾丸。比较显示，产后 11 天时，生殖细胞的形态和数量没有显著差异，而鉴定出与精子发生相关的 21 个差异表达基因（DEGs）。我们推测 Stra8 通过直接或间接调节这些 DEGs，在精子发生的不同阶段发挥许多功能，例如精原干细胞的建立、精原细胞的增殖和自我更新、精原细胞的分化和减数分裂。因此，我们建立了 Stra8 在精子发生过程中的初步调控网络。这些结果将为进一步研究 Stra8 在精子发生中的作用机制提供理论基础。
