Mitsui Yoshinori, Kato Kentaro
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University.
Department of Eco-epidemiology, Institute of Tropical Medicine, Nagasaki University.
Jpn J Infect Dis. 2018 Sep 21;71(5):382-387. doi: 10.7883/yoken.JJID.2018.034. Epub 2018 May 31.
The possible emergence of praziquantel (PZQ)-tolerant and/or -resistant schistosomes requires the study and development of new antischistosomal drugs as alternatives to PZQ. The present study investigates the capability of 3 dyes-methylene blue (MB), neutral red (NR), and trypan blue (TB)-to assess the in vitro antischistosomal effect of antimalarial drugs on Schistosoma mansoni adult worms. S. mansoni adult worms were incubated in the medium alone as the control or in the medium supplemented with 10 μg/ml primaquine (PQ), artesunate (AR), or amodiaquine (AQ) for 5 days. Viabilities of the worms were observed following staining with MB, NR, or TB. The disparity of MB and NR staining among male and female adult worms treated with PQ, AR, and AQ correlated with the various levels of damage to the male and female worms. Furthermore, the severity of the damage to the adult worms treated with the 3 drugs appeared to be reflected in the TB staining status. The results indicate that the 3 non-fluorescent dyes can serve as useful complementary tools to assess the antischistosomal effect of antimalarial drugs.
可能出现对吡喹酮（PZQ）耐受和/或耐药的血吸虫，这就需要研究和开发新的抗血吸虫药物作为PZQ的替代品。本研究调查了3种染料——亚甲蓝（MB）、中性红（NR）和台盼蓝（TB）——评估抗疟药物对曼氏血吸虫成虫的体外抗血吸虫作用的能力。将曼氏血吸虫成虫单独置于培养基中作为对照，或置于添加了10μg/ml伯氨喹（PQ）、青蒿琥酯（AR）或阿莫地喹（AQ）的培养基中培养5天。用MB、NR或TB染色后观察虫体活力。用PQ、AR和AQ处理的雌雄成虫之间MB和NR染色的差异与雌雄虫体不同程度的损伤相关。此外，用这3种药物处理的成虫的损伤严重程度似乎反映在TB染色状态上。结果表明，这3种非荧光染料可作为评估抗疟药物抗血吸虫作用的有用补充工具。
