Matsuura Takashi, Sugimoto Kouji, Kawata-Matsuura Viviane K S, Yanagiguchi Kajiro, Yamada Shizuka, Hayashi Yoshihiko
Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences.
Department of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences.
J Oral Sci. 2018 Dec 29;60(4):634-637. doi: 10.2334/josnusd.17-0456. Epub 2018 May 31.
Once a tooth develops deep caries and the dental pulp tissue is irreversibly infected, the infected dental pulp tissue should be removed, and filling material should be placed in the root canal. Endodontically treated teeth are prone to root fracture or periapical periodontitis; however, dental pulp tissue has the potential to prevent root fracture or periapical periodontitis. Therefore, dental pulp regeneration after pulpectomy may help prolong tooth life. In this study, a new method of dental pulp regeneration was developed. Vascular endothelial growth factor-adsorbed collagen gel was injected into the root canal of a prepared root canal model, placed into the dorsum of a rat, and cultured for 3 weeks. After retrieving the implant, histological analysis was performed. It was found that rat somatic cells were recruited into the root apex of the transplanted root canal model. These findings suggest a new potential technique for engineering dental pulp tissue.
一旦牙齿发生深龋且牙髓组织受到不可逆感染，就应去除受感染的牙髓组织，并在根管内放置填充材料。经过牙髓治疗的牙齿容易发生牙根折断或根尖周炎；然而，牙髓组织具有预防牙根折断或根尖周炎的潜力。因此，牙髓切除术后的牙髓再生可能有助于延长牙齿寿命。在本研究中，开发了一种新的牙髓再生方法。将血管内皮生长因子吸附的胶原凝胶注入制备好的根管模型的根管内，置于大鼠背部并培养3周。取出植入物后，进行组织学分析。发现大鼠体细胞被募集到移植根管模型的根尖。这些发现提示了一种用于构建牙髓组织的新的潜在技术。
