George Mosotho J, Njobeh Patrick B, Gbashi Sefater, Adegoke Gabriel O, Dubery Ian A, Madala Ntakadzeni E
Department of Chemistry and Chemical Technology, National University of Lesotho, Roma 180, Lesotho.
Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South Africa.
Int J Anal Chem. 2018 Apr 19;2018:8976304. doi: 10.1155/2018/8976304. eCollection 2018.
Volatile organic compounds (VOCs) derived from plants have been used in the fragrance industry since time immemorial. Herein we report on the rapid screening of VOCs from seeds of ripe (family, Zingiberaceae) using a polydimethylsiloxane fibre headspace solid phase microextraction coupled to a gas chromatography mass spectrometry (SPME-GC/MS) instrument. Portions of 0.25, 0.35, and 0.50 g of ground sample were weighed and extraction of volatile organic compounds (VOCs) was achieved using a 100 m polydimethylsiloxane solid phase microextraction (PDMS SPME) fibre, with the equilibrium time of 40 minutes and extraction temperature of 50°C; the following compounds with their respective relative abundances were obtained as the top ten most abundant and annotated ones using NIST, Wiley, and Fragrances Libraries: eucalyptol (58%); -pinene (22%); -pinene (7.5%); -terpineol (4%), -terpinyl acetate (2%); -bergamotene (1%); pinocarveol (0.39%); -copaene (0.35%); caryophyllene (0.34); and -bisabolene (0.31%). These compounds have been reported elsewhere in the literature and listed in the Fragrances Library, incorporated into the Saturn QP2020 GCMS Solution® software used for their analysis.
源自植物的挥发性有机化合物（VOCs）自古以来就被用于香料工业。在此，我们报告了使用聚二甲基硅氧烷纤维顶空固相微萃取与气相色谱-质谱联用仪（SPME-GC/MS）对成熟的姜科植物种子中的挥发性有机化合物进行快速筛选的情况。称取0.25、0.35和0.50克研磨后的样品，使用100米聚二甲基硅氧烷固相微萃取（PDMS SPME）纤维萃取挥发性有机化合物（VOCs），平衡时间为40分钟，萃取温度为50°C；使用NIST、Wiley和香料库获得了以下各自相对丰度的化合物，作为含量最高的前十种且已注释的化合物：桉叶油素（58%）；α-蒎烯（22%）；β-蒎烯（7.5%）；γ-萜品醇（4%），乙酸γ-萜品酯（2%）；β-香柠檬烯（1%）；松芹醇（0.39%）；α-可巴烯（0.35%）；石竹烯（0.34%）；以及β-红没药烯（0.31%）。这些化合物在其他文献中已有报道，并列入了香料库，纳入了用于分析的Saturn QP2020 GCMS Solution®软件中。
