重组DNA技术在α1-抗胰蛋白酶变体诊断和研究中的可能应用。

Possible applications of recombinant DNA technology to the diagnosis and study of variants of alpha 1-antitrypsin.

作者信息

Kalsheker N, Chiswell D, Markham A, Imam A, Wallis S, Williamson R, Humphries S E

出版信息

Ann Clin Biochem. 1985 Jan;22 ( Pt 1):25-32. doi: 10.1177/000456328502200102.

DOI:10.1177/000456328502200102

Abstract

We describe the basic principles of recombinant DNA technology and how this methodology has been used to isolate and characterise cloned genes coding for alpha 1-antitrypsin. We show how these probes can be used for diagnosis and to study molecular variants of alpha 1-antitrypsin which may predispose individuals to develop lung disease.

摘要

我们描述了重组DNA技术的基本原理，以及该方法如何用于分离和鉴定编码α1-抗胰蛋白酶的克隆基因。我们展示了这些探针如何用于诊断以及研究α1-抗胰蛋白酶的分子变体，这些变体可能使个体易患肺部疾病。