评估从伊朗亚兹德住院患者中分离出的耐氨基糖苷类菌株中 、 、 和 基因的频率。
Evaluating the Frequency of , , , and Genes in Aminoglycosides Resistant Isolates Obtained from Hospitalized Patients in Yazd, Iran.
作者信息
Mokhtari Hesam, Eslami Gilda, Zandi Hengameh, Dehghan-Banadkouki Amin, Vakili Mahmood
机构信息
International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Avicenna J Med Biotechnol. 2018 Apr-Jun;10(2):115-119.
BACKGROUND
is an opportunistic pathogen that could be resistant to many antimicrobial agents. Resistance genes can be carried among gram-negative bacteria by integrons. Enzymatic inactivation is the most important mechanism of resistance to aminoglycosides. In this study, the frequencies of two important resistance gene a and and genes coding integrase I and II, in isolates resistant to aminoglycosides were evaluated.
METHODS
In this cross-sectional study, an attempt was made to assess the antibiotic susceptibility of 130 isolates obtained from different samples of patients hospitalized in training hospitals of Yazd evaluated by disk diffusion method. The frequencies of a, , and genes were determined by PCR method. Data were analyzed by chi-square method using SPSS software (Ver. 16).
RESULTS
our results showed that resistance to gentamicin, tobramycin, kanamycin, and amikacin were 34.6, 33.8, 43.8, and 14.6%, respectively. The frequencies of a, , and genes were 44.6, 27.7, 90, and 0%, respectively.
CONCLUSION
This study showed there are high frequencies of genes coding aminoglycosides resistance in isolates. Hence, it is very important to monitor and inhibit the spread of antibiotic resistance genes.
背景
是一种机会性病原体，可能对多种抗菌药物耐药。耐药基因可通过整合子在革兰氏阴性菌之间携带。酶促失活是对氨基糖苷类耐药的最重要机制。在本研究中，评估了对氨基糖苷类耐药的分离株中两个重要耐药基因a和以及编码整合酶I和II的基因的频率。
方法
在这项横断面研究中，尝试通过纸片扩散法评估从亚兹德培训医院住院患者的不同样本中获得的130株分离株的抗生素敏感性。通过PCR方法确定a、和基因的频率。使用SPSS软件（版本16）通过卡方检验分析数据。
结果
我们的结果显示，对庆大霉素、妥布霉素、卡那霉素和阿米卡星的耐药率分别为34.6%、33.8%、43.8%和14.6%。a、和基因的频率分别为44.6%、27.7%、90%和0%。
结论
本研究表明，分离株中编码氨基糖苷类耐药的基因频率很高。因此，监测和抑制抗生素耐药基因的传播非常重要。
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