Mehrabi M, Salehi B, Rassi H, Dehghan A
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
New Microbes New Infect. 2020 Nov 26;38:100827. doi: 10.1016/j.nmni.2020.100827. eCollection 2020 Nov.
The present paper aims to determine the frequency and antibiotic resistance patterns of pathogenic bacteria, the virulence factor profile of and mannose-binding lectin (MBL) gene polymorphism in individuals with diabetes mellitus (DM) and urinary tract infection (UTI). The population under study was 130 individuals with type 2 diabetes mellitus (T2DM) and UTI. The patients' clinical characteristics and urine and blood samples (5 mL) were collected. Antibiotic resistance was determined using a disc diffusion method, and the results were interpreted according to CLSI. The presence of virulence genes was detected by multiplex PCR. To detect the MBL gene polymorphism, PCR and restriction fragment length polymorphism methods were applied. The predominant Gram-negative and Gram-positive bacteria included and spp.viridans group, respectively. Women were more susceptible to the incidence of UTI than men. The isolates showed a high level of resistance to amoxicillin-clavulanic acid (87.35%), and nitrofurantoin and ceftizoxime were the most effective antimicrobial agents for . Cefotaxime and ceftizoxime were the most effective antimicrobial agents for spp., norfloxacin and ciprofloxacin were the most effective antimicrobial agents for and (52.87%) and (1.14%) had the highest and lowest frequency among examined genes in isolates, respectively. The GG genotype had the highest frequency among patients with T2DM and UTI. Results showed that the detection of in individuals with an AA genotype, codon 54 of the MBL gene, can play an important role in the molecular diagnosis and timely treatment of bacterial infections in individuals with diabetes.
本文旨在确定糖尿病（DM）合并尿路感染（UTI）患者中病原菌的频率和抗生素耐药模式、毒力因子谱以及甘露糖结合凝集素（MBL）基因多态性。研究人群为130例2型糖尿病（T2DM）合并UTI患者。收集患者的临床特征以及尿液和血液样本（5毫升）。采用纸片扩散法测定抗生素耐药性，并根据美国临床和实验室标准协会（CLSI）的标准解释结果。通过多重聚合酶链反应（PCR）检测毒力基因的存在。为检测MBL基因多态性，应用了PCR和限制性片段长度多态性方法。主要的革兰氏阴性菌和革兰氏阳性菌分别为大肠埃希菌和草绿色链球菌。女性比男性更容易发生UTI。大肠埃希菌分离株对阿莫西林-克拉维酸表现出高水平耐药（87.35%），呋喃妥因和头孢唑肟是对大肠埃希菌最有效的抗菌药物。头孢噻肟和头孢唑肟是对草绿色链球菌最有效的抗菌药物，诺氟沙星和环丙沙星是对粪肠球菌和屎肠球菌最有效的抗菌药物，在大肠埃希菌分离株中， examined基因中分别有最高和最低频率。GG基因型在T2DM合并UTI患者中频率最高。结果表明，在MBL基因第54密码子AA基因型个体中检测该基因，可在糖尿病个体细菌感染的分子诊断和及时治疗中发挥重要作用。
