de Paolis A, Chandra S, Charalambides A A, Bonnett R, Magnus I A
Biochem J. 1985 Mar 15;226(3):757-66. doi: 10.1042/bj2260757.
A comparison of the photosensitizing ability of a variety of porphyrins for photohaemolysis gives the following order of activity: protoporphyrin greater than deuteroporphyrin, mesoporphyrin, haematoporphyrin dimethyl ester much greater than haematoporphyrin diacetate, haematoporphyrin greater than haematoporphyrin monoacetate, coproporphyrin III, haematoporphyrin derivative, coproporphyrin III tetramethyl ester greater than uroporphyrin I, meso-tetra-(N-methyl-4-pyridinium)porphyrin tetratoluene-p-sulphonate, meso-tetra-(p-carboxyphenyl)porphyrin, protoporphyrin dimethyl ester, meso-tetra-(p-hydroxy-sulphonylphenyl)porphyrin tetrasodium salt, uroporphyrin III, deuteroporphyrin-3,8-disulphonic acid and protohaemin. The results for the metal-free porphyrins are rationalized in terms of solubility and partition properties, and a model is proposed for the incorporation of amphipathic porphyrins into the membrane lipid bilayer. Experiments with erythrocytes from patients with erythropoeitic protoporphyria and with normal erythrocytes to which porphyrin was added in a deuterium oxide medium do not lead to an increase in the rate of photohaemolysis. A possible explanation for this somewhat surprising observation is outlined.
对多种卟啉的光溶血光敏化能力进行比较，得到以下活性顺序：原卟啉大于次卟啉、中卟啉、血卟啉二甲酯远大于血卟啉二乙酸酯、血卟啉大于血卟啉单乙酸酯、粪卟啉III、血卟啉衍生物、粪卟啉III四甲酯大于尿卟啉I、中-四-（N-甲基-4-吡啶鎓）卟啉四甲苯磺酸盐、中-四-（对羧基苯基）卟啉、原卟啉二甲酯、中-四-（对羟基磺酰苯基）卟啉四钠盐、尿卟啉III、次卟啉-3,8-二磺酸和原血红素。无金属卟啉的结果根据溶解度和分配性质进行了合理化解释，并提出了一个将两亲性卟啉纳入膜脂双层的模型。对红细胞生成性原卟啉症患者的红细胞以及在重水介质中添加了卟啉的正常红细胞进行的实验，并未导致光溶血速率增加。文中概述了对这一有点令人惊讶的观察结果的一种可能解释。
