Experiment Centre of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Biomed Res Int. 2018 Apr 12;2018:2079195. doi: 10.1155/2018/2079195. eCollection 2018.
QM9414, 3.316, M85, and M92 were screened for hydrolyzing geniposide into genipin. was selected according to the -glucosidase activity of the fermentation broths using geniposide as a substrate. was immobilized by embedding method using sodium alginate as the carrier. Geniposide was hydrolyzed by immobilized at 28°C (200 rpm) for 34 h, and the yield of genipin was 89%. The product was purified and identified by UV, IR, EIMS, and H-NMR. Since there were two sets of signals in H-NMR spectra, a series of experiments were performed and verified that the existence of two conformations was the main reason. Generally, biotransformation of geniposide into genipin by immobilized provides a promising solution to the genipin production.
QM9414、3.316、M85 和 M92 被筛选用于将京尼平苷水解成京尼平。根据发酵液中以京尼平苷为底物的β-葡萄糖苷酶活性，选择了。通过使用海藻酸钠作为载体的包埋法固定化。在 28°C（200rpm）下用固定化酶水解京尼平苷 34h，京尼平的产率为 89%。通过 UV、IR、EIMS 和 H-NMR 对产物进行了纯化和鉴定。由于 H-NMR 图谱中有两组信号，因此进行了一系列实验并验证了两种构象的存在是主要原因。一般来说，固定化酶转化京尼平苷生成京尼平为京尼平的生产提供了一种很有前途的解决方案。
