Mayrand D, Grenier D
Can J Microbiol. 1985 Feb;31(2):134-8. doi: 10.1139/m85-026.
Collagenolytic activity of 12 species of oral bacteria was assessed using two methods of detection. Except for two species, all bacterial strains tested were capable of degrading at least one general protein substrate. Results of collagenolytic activity in a growth assay indicate that Bacteroides gingivalis is the only bacterium capable of degrading collagen when the substrate is sterilized using ethylene oxide. However, if the substrate is sterilized by autoclaving, in the presence or absence of the growth medium, other bacterial species could be shown to be collagenolytic. Collagenolytic activity was also demonstrated when whole or broken cells were used in a [14C]collagen assay. Results from this assay and from inhibition studies indicate that collagenolytic activity can either be the result of the combined activities of both a specific collagenase and nonspecific proteases (B. gingivalis) or nonspecific proteases only (other strains in this study), although in the latter case, the time taken to hydrolyze collagen can be 10 times longer than with a specific collagenase.
使用两种检测方法评估了12种口腔细菌的胶原酶活性。除两种细菌外，所有测试菌株均能够降解至少一种通用蛋白质底物。生长试验中的胶原酶活性结果表明，当底物用环氧乙烷灭菌时，牙龈卟啉单胞菌是唯一能够降解胶原的细菌。然而，如果底物通过高压灭菌进行灭菌，无论有无生长培养基，其他细菌种类也可表现出胶原酶活性。当完整或破碎的细胞用于[14C]胶原试验时，也证明了胶原酶活性。该试验结果和抑制研究表明，胶原酶活性可能是特定胶原酶和非特异性蛋白酶共同作用的结果（牙龈卟啉单胞菌），也可能仅是非特异性蛋白酶的作用结果（本研究中的其他菌株），尽管在后一种情况下，水解胶原所需的时间可能比使用特定胶原酶时长10倍。
