Sever David M, Rania Lisa C, Krenz John D
Department of Biology, Saint Mary's College, Notre Dame, Indiana 46556.
Savannah River Ecology Laboratory, Drawer E, Aiken, South Carolina 29802.
J Morphol. 1996 Feb;227(2):155-170. doi: 10.1002/(SICI)1097-4687(199602)227:2<155::AID-JMOR3>3.0.CO;2-8.
Female sperm storage was studied in a population of Notophthalmus viridescens from South Carolina. Spermathecae initiate production of a glycoprotein secretory product in October. At this time ovarian follicles are immature (0.5-0.9 mm dia), and mating does not occur despite spermiation in males. Six of the 10 females collected in December had sperm in their spermathecae, indicating onset of mating. Unmated females collected in October and sacrificed in February and March possessed mature ovarian follicles (1.3-1.4 mm dia), and the spermathecae contained large secretory vacuoles 2-3 μm dia. Release of secretory product is concomitant with the appearance of sperm in the spermathecae. Thus mated females lack secretory vacuoles in the spermathecal epithelium, and additional synthesis of secretory product does not occur. All females collected in February and March have mated. Sperm are embedded in the spermathecal epithelium and are undergoing degradation in February. Degradation of sperm in the lumen and epithelium is evident in specimens examined from May and June. Atresia of ovarian follicles begins in April in captive specimens, and specimens captured from the bay in May are spent. A general postbreeding emigration from the pond occurs in summer. Fourteen females collected 7 March were injected with human chorionic gonadotropin (hCG) on 9 March and laid fertile eggs 10-18 March. Two of these females were sacrificed each month from April-September; all retained some sperm in their spermathecae, but further oviposition did not occur. Four females were kept 1 year after oviposition of fertile eggs, and oviposition again was induced with hCG; these eggs were infertile, and spermathecae lacked sperm. Spermathecae are inactive from June-September in captive and wild-caught specimens. Sperm may be stored effectively up to 6 months (December-May), and no evidence was found for retention of viable sperm from one breeding season to the next. © 1996 Wiley-Liss, Inc.
对来自南卡罗来纳州的一群绿红东美螈进行了雌性精子储存的研究。受精囊在10月开始产生一种糖蛋白分泌产物。此时卵巢卵泡不成熟（直径0.5 - 0.9毫米），尽管雄性有排精现象，但交配并未发生。12月收集的10只雌性中有6只的受精囊中含有精子，表明开始交配。10月收集并于2月和3月处死的未交配雌性拥有成熟的卵巢卵泡（直径1.3 - 1.4毫米），受精囊中含有直径2 - 3微米的大分泌泡。分泌产物的释放与受精囊中精子的出现同时发生。因此，已交配的雌性受精囊上皮中没有分泌泡，也不再额外合成分泌产物。2月和3月收集的所有雌性都已交配。精子嵌入受精囊上皮，并在2月开始降解。在5月和6月检查的标本中，管腔和上皮中的精子降解明显。圈养标本的卵巢卵泡闭锁在4月开始，5月从海湾捕获的标本已无生殖能力。夏季一般会有从池塘的繁殖后迁徙。3月7日收集的14只雌性在3月9日注射了人绒毛膜促性腺激素（hCG），并于3月10日至18日产下可育卵。从4月到9月，每月处死其中2只雌性；所有雌性的受精囊中都保留了一些精子，但未再产卵。4只雌性在产下可育卵后饲养1年，再次用hCG诱导产卵；这些卵不育，受精囊中也没有精子。在圈养和野生捕获的标本中，受精囊在6月至9月不活跃。精子可有效储存长达6个月（12月至5月），未发现有存活精子从一个繁殖季节保留到下一个繁殖季节的证据。© 1996威利 - 利斯公司。
