Sever David M, Hamlett William C
Department of Biology, Saint Mary's College, Notre Dame, Indiana.
Indiana University School of Medicine, South Bend Center for Medical Education, Notre Dame, Indiana.
J Morphol. 1998 Nov;238(2):143-155. doi: 10.1002/(SICI)1097-4687(199811)238:2<143::AID-JMOR2>3.0.CO;2-R.
The alignment of sperm in a cloacal sperm storage gland, the spermatheca, was studied in female desmognathine salamanders by scanning and transmission electron microscopy. Females representing nine species and collected in spring, late summer, and fall in the southern Appalachian Mountains contained abundant sperm in their spermathecae. The spermatheca is a compound tubuloalveolar gland connected by a single common tube to the middorsal wall of the cloaca. Sperm enter the common tube in small groups aligned in parallel along their axes, and continue in a straight course until encountering divisions of the common tube (neck tubules) or luminal borders of distal bulbs, which can act as barriers. Sperm may form tangles, in which small clusters retain their mutual alignment, at the branches of the neck tubules from the common tube, or in the lumen of the distal bulbs, where subsequent waves of sperm collide with sperm already present. The nuclei of some sperm from the initial group to encounter the walls of the distal bulbs appear to become embedded in secretory material on the luminal border or in the apical cytoplasm of the spermathecal epithelial cells. We propose that these sperm become trapped in the spermatheca and are ultimately degraded. J. Morphol. 238:143-155, 1998. © 1998 Wiley-Liss, Inc.
