School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Center, New York University School of Medicine, 522 First Avenue, Smilow Research Building 1107, New York, NY 10016, United States.
Talanta. 2018 Sep 1;187:265-271. doi: 10.1016/j.talanta.2018.05.037. Epub 2018 May 9.
A new non-enzyme method based on hybridization chain reaction (HCR) and colorimetric reaction catalyzed by magnetic Ni/Fe layered double hydroxide (LDH) nanosheets was developed for detection of microRNA (miRNA), let-7b. The DNA hairpins from HCR were separated and adsorbed by Ni/Fe LDH. The peroxidase-like activity of Ni/Fe LDH was found to be enhanced by the DNA hairpins on the surface. The factors, such as ratio of Ni/Fe and concentration of DNA hairpins, related to the catalytic activity were evaluated and the mechanism was discussed. The results of this new detection method for let-7b provided low limit of detection (0.36 fM), wide linear range (0.01 pM to 200 pM) with good linearity (r = 0.9968). The optimized method was applied to analyze let-7b in real samples, lung cancer cells. This work demonstrated a new and cost-effective approach for efficient detection of miRNA.
基于杂交链式反应（HCR）和磁性 Ni/Fe 层状双氢氧化物（LDH）纳米片催化的比色反应，开发了一种新的非酶方法，用于检测 microRNA（miRNA），let-7b。HCR 的 DNA 发夹通过 Ni/Fe LDH 分离和吸附。发现 Ni/Fe LDH 的过氧化物酶样活性通过表面上的 DNA 发夹得到增强。评估了与催化活性相关的 Ni/Fe 比例和 DNA 发夹浓度等因素，并讨论了其机制。该方法用于检测 let-7b 的结果提供了低检测限（0.36 fM）、宽线性范围（0.01 pM 至 200 pM）和良好的线性度（r = 0.9968）。优化后的方法用于分析真实样品中的 let-7b，即肺癌细胞。这项工作展示了一种新的、具有成本效益的方法，可有效检测 miRNA。
