Guyasa Babe, Melaku Yadessa, Endale Milkyas
Applied Chemistry Program, School of Applied Natural Science, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia.
Adv Pharmacol Sci. 2018 Apr 30;2018:5870161. doi: 10.1155/2018/5870161. eCollection 2018.
Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3',4'-pentahydroxyflavan, named epicatechin (), along with a close flavan derivative (). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for antibacterial activity against strains of , , , , and . Epicatechin () exhibited comparable antibacterial activity against and (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 g/mL.
瓦特克是一种传统上用于治疗肠道绦虫、痛经、细菌和真菌感染的药用植物。对二氯甲烷/甲醇（1∶1）提取物和甲醇提取物进行的植物化学筛选试验显示，其中存在酚类、生物碱、单宁和黄酮类化合物，而萜类、糖苷和植物甾醇不存在。甲醇提取物经硅胶柱色谱分离得到3,5,7,3',4'-五羟基黄烷，即表儿茶素（），以及一种与之相近的黄烷衍生物（）。通过光谱技术（一维和二维核磁共振、傅里叶变换红外光谱和紫外可见光谱）阐明了这些化合物的结构。对粗提取物和分离出的化合物针对、、、和菌株进行了抗菌活性筛选。表儿茶素（）对和表现出与对照抗生素庆大霉素相当的抗菌活性（抑菌圈分别为15和12毫米），在浓度为20微克/毫升时，抑菌圈分别为15和12毫米。
