钙蛋白酶I（低钙依赖性半胱氨酸蛋白酶的低钙需求形式）在人胰岛B细胞中的选择性定位。
Selective localization of calpain I (the low-Ca2+-requiring form of Ca2+-dependent cysteine proteinase) in B-cells of human pancreatic islets.
作者信息
Kitahara A, Ohtsuki H, Kirihata Y, Yamagata Y, Takano E, Kannagi R, Murachi T
出版信息
FEBS Lett. 1985 May 6;184(1):120-4. doi: 10.1016/0014-5793(85)80666-9.
An immunohistochemical study was performed to localize two distinct Ca2+-proteases (low-Ca2+-requiring calpain I and high-Ca2+-requiring calpain II) and their specific inhibitor (calpastatin) in human pancreas using the respective monospecific antibodies. Strongly positive staining by anti-calpain I antibody was found in pancreatic islets, specifically in B-cells, whereas the exocrine pancreatic tissue showed essentially no positive immunostaining. No such specific staining was found with anti-calpain II antibodies or anti-calpastatin antibodies. The results suggest that the Ca2+-dependent proteolysis in B-cells can be triggered by a small rise of the intracellular Ca2+ concentration without serious interference by the endogenous inhibitor.
利用各自的单特异性抗体进行免疫组织化学研究，以在人胰腺中定位两种不同的钙蛋白酶（低钙需求的钙蛋白酶I和高钙需求的钙蛋白酶II）及其特异性抑制剂（钙蛋白酶抑制蛋白）。抗钙蛋白酶I抗体染色显示，胰岛，尤其是B细胞呈强阳性，而胰腺外分泌组织基本无阳性免疫染色。抗钙蛋白酶II抗体或抗钙蛋白酶抑制蛋白抗体未发现此类特异性染色。结果表明，B细胞内的钙依赖性蛋白水解可由细胞内钙浓度的小幅升高触发，而不受内源性抑制剂的严重干扰。
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