环磷酸腺苷诱导肾小球加压素的产生。

Induction of glomerulopressin production by cyclic AMP.

作者信息

Arany E, Uranga J, Bonetto R, del Castillo E

出版信息

Horm Metab Res. 1985 Feb;17(2):72-7. doi: 10.1055/s-2007-1013455.

DOI:10.1055/s-2007-1013455

Abstract

Isolated rat livers were perfused with gassed Krebs-Ringer-Bicarbonate and different doses of theophylline and dibutyryl cyclic AMP were added to the perfusing solution. The perfusates were ultrafiltrated through Diaflo UM-05 membranes. The glomerulopressin activity of the ultrafiltrates were assayed in the tonic tension contraction (TTC) of isolated stomach fundus from rats. As glomerulopressin is known to be a glucuronide, it was inactivated with beta-glucuronidase to confirm that the effect on the stomach fundus was due to the glomerulopressin and not to another substance. It was observed that doses of theophylline between 2 x 10(-3) M and 2 x 10(-5) M enhanced glomerulopressin production. However, there was no relationship between dose of theophylline and the response, and a dose of theophylline 2 x 10(-6) M has no activity. The perfusion with dibutyryl cyclic AMP at 5 x 10(-8) M increased the amount of glomerulopressin produced by the liver. This was a log-dose response of glomerulopressin production to dibutyryl cyclic AMP between 5 x 10(-8) M and 5 x 10(-4) M. Theophylline (2 x 10(-6) M) potentiated the activity of cyclic AMP (5 x 10(-8) M). These results support the view that cyclic AMP is intracellular mediator of the hepatic production of glomerulopressin.

摘要

用充氧的 Krebs - Ringer - 碳酸氢盐灌注离体大鼠肝脏，并向灌注液中添加不同剂量的茶碱和二丁酰环磷腺苷。灌注液通过 Diaflo UM - 05 膜进行超滤。在大鼠离体胃底的强直张力收缩（TTC）中测定超滤物的肾小球加压素活性。由于已知肾小球加压素是一种葡糖醛酸苷，所以用β - 葡糖醛酸酶使其失活，以确认对胃底的作用是由肾小球加压素引起的，而非其他物质。观察到茶碱剂量在 2×10⁻³ M 至 2×10⁻⁵ M 之间可增强肾小球加压素的产生。然而，茶碱剂量与反应之间并无关联，2×10⁻⁶ M 的茶碱剂量无活性。用 5×10⁻⁸ M 的二丁酰环磷腺苷灌注可增加肝脏产生的肾小球加压素量。这是肾小球加压素产生量对二丁酰环磷腺苷在 5×10⁻⁸ M 至 5×10⁻⁴ M 之间的对数剂量反应。茶碱（2×10⁻⁶ M）增强了环磷腺苷（5×10⁻⁸ M）的活性。这些结果支持环磷腺苷是肝脏产生肾小球加压素的细胞内介质这一观点。